ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSNNPDNNN SNNINNNNKD KDIAPNSDVQ LATVYTKAKS YIPQIEQVYQ GTNPNIQEAK 

        70         80         90        100        110        120 
LLGELLQVLA EVPKGTHLFC DPILEPISIF SLTIFSFNEE ATATWLKNHF NPILSVCDKC 

       130        140        150        160        170        180 
ILNFARGKCK MLQHFAIQRH VPHEHVAKFN DIVCQWRVEA VFPILRNISV NDNTGINITN 

       190        200        210        220        230        240 
EIETAMYECL CNPHMLRLNK QLKATFEAIF KFFYDTKHRL LDVTNPLSIK TFISGVIFCW 

       250        260        270        280        290        300 
CEGSKEENEW SRAFLKDLYS RNFHINLSNL TPDIIEEVYI HILFLQNPAN WTEIVVSQFW 

       310        320        330        340        350        360 
SRLLPVFNLF DKDVFIEYFQ VPKNVESLKK TFKFPLEPIF KMWYNHLSKS YHDKPLDFLL 

       370        380        390        400        410        420 
RGLTMFLNKF GSEFWSKIEP FTFHSILDII FNRDSFPIKL IKIQDNPIVE HQTEVYFQLT 

       430        440        450        460        470        480 
GSVTDLLSWT LPFYHALSPS KRIQMVRKVS MAFLRIIANY PSLKSIPKAC LMNSATALLR 

       490        500        510        520        530        540 
AVLTIKENER AMLYKNDEFE TVLLTKTDSR ALLNNPLIQD IIIRSASNPN DFYPGLGAAS 

       550        560        570        580        590        600 
ASVATSTMMV LAECIDFDIL LLCHRTFKLY SGKPISEIPI STNVLENVTN KIDLRSFHDG 

       610        620        630        640        650        660 
PLLAKQLLVS LKNINGLLIV PSNTAVAEAH NALNQKFLLL STRLMEKFAD ILPGQLSKIL 

       670        680        690        700        710        720 
ADEDASQGFW SCIFSSDKHL YQAATNILYN TFDVEGRLEG ILAILNSNLT VNLKNINVML 

       730        740        750        760        770        780 
QRLINCEFYE PCPRAVRVLM DVVSAFVDPI SGVFANFQTL KSQNTEKEFL KFWESCWLFL 

       790        800        810        820        830        840 
DTIYKFTLKW ASKYDYSELE NFTKDTLDLS RSLVDSFREF SDILHDQTKN LLLNVLETFK 

       850        860        870        880        890        900 
NMLYWLRLSD EVLLESCVRL IISTSDLAHE KHVKVDDSLV EMMAKYASKA KRFSNKLTEQ 

       910        920        930        940        950        960 
QASEILQKAK IFNKALTEEV ATEAENYRKE KELSRLGKVI DLTDSVPASP SLSPSLSSTI 

       970        980        990       1000       1010       1020 
ASSSAESRAD YLQRKALSSS ITGRPRVAQP KITSFGTFQS SANAKLHRTK PVKPLSKMEL 

      1030       1040       1050       1060       1070       1080 
ARMQLLNNRV VHPPSAPAFH TKSRGLSNKN DDSSSEESDN DIESARELFA IAKAKGKGIQ 

      1090       1100       1110       1120       1130       1140 
TVDINGKVVK RQTAAELAKQ ELEHMRKRLN VDMNPLYEII LQWDYTRNSE YPDDEPIGNY 

      1150       1160       1170       1180       1190       1200 
SDVKDFFNSP ADYQKVMKPL LLLESWQGLC SSRDREDYKP FSIIVGNRTA VSDFYDVYAS 

      1210       1220       1230       1240       1250       1260 
VAKQVIQDCG ISESDLIVMA YLPDFRPDKR LSSDDFKKAQ HTCLAKVRTL KNTKGGNVDV 

      1270       1280       1290       1300       1310       1320 
TLRIHRNHSF SKFLTLRSEI YCVKVMQMTT IEREYSTLEG LEYYDLVGQI LQAKPSPPVN 

      1330       1340       1350       1360       1370       1380 
VDAAEIETVK KSYKLNTSQA EAIVNSVSKE GFSLIQGPPG TGKTKTILGI IGYFLSTKNA 

      1390       1400       1410       1420       1430       1440 
SSSNVIKVPL EKNSSNTEQL LKKQKILICA PSNAAVDEIC LRLKSGVYDK QGHQFKPQLV 

      1450       1460       1470       1480       1490       1500 
RVGRSDVVNV AIKDLTLEEL VDKRIGERNY EIRTDPELER KFNNAVTKRR ELRGKLDSES 

      1510       1520       1530       1540       1550       1560 
GNPESPMSTE DISKLQLKIR ELSKIINELG RDRDEMREKN SVNYRNRDLD RRNAQAHILA 

      1570       1580       1590       1600       1610       1620 
VSDIICSTLS GSAHDVLATM GIKFDTVIID EACQCTELSS IIPLRYGGKR CIMVGDPNQL 

      1630       1640       1650       1660       1670       1680 
PPTVLSGAAS NFKYNQSLFV RMEKNSSPYL LDVQYRMHPS ISKFPSSEFY QGRLKDGPGM 

      1690       1700       1710       1720       1730       1740 
DILNKRPWHQ LEPLAPYKFF DIISGRQEQN AKTMSYTNME EIRVAIELVD YLFRKFDNKI 

      1750       1760       1770       1780       1790       1800 
DFTGKIGIIS PYREQMQKMR KEFARYFGGM INKSIDFNTI DGFQGQEKEI ILISCVRADD 

      1810       1820       1830       1840       1850       1860 
TKSSVGFLKD FRRMNVALTR AKTSIWVLGH QRSLAKSKLW RDLIEDAKDR SCLAYACSGF 

      1870       1880       1890       1900       1910       1920 
LDPRNNRAQS ILRKFNVPVP SEQEDDYKLP MEYITQGPDE VKSNKDTKKR RVVDEGEEAD 

      1930       1940       1950       1960       1970       1980 
KAVKKKKKEK KKEKKKSKAD DKKKNNKKAE SPSTSSGTKK KSSIFGGMSV PSAVVPKTFP 

      1990       2000       2010       2020       2030       2040 
DVDSNKKAAA VVGKKKNNKH VCFSDDVSFI PRNDEPEIKV TRSLSSVLKE KQLGLKETRT 

      2050       2060       2070       2080       2090       2100 
ISPPEISNNE DDDDEDDYTP SISDSSLMKS EANGRNNRVA SHNQNFSASI YDDPQVSQAK 

      2110       2120       2130       2140       2150       2160 
QTQVPAAITK HRSSNSVLSG GSSRILTASD YGEPNQNGQN GANRTLSQHV GNANQYSTAP 

      2170       2180       2190       2200       2210       2220 
VGTGELHETL PAHPQDSYPA EAEDPYDLNP HPQPQSSAFK GPGSGPTGTR NSSRRNASSS 

      2230 
PFIPKKRKPR S 

References and documentation are available.

Number of amino acids: 2231

Molecular weight: 252497.13

Theoretical pI: 8.78

Amino acid composition: 
Ala (A) 134	  6.0%
Arg (R) 110	  4.9%
Asn (N) 145	  6.5%
Asp (D) 133	  6.0%
Cys (C)  31	  1.4%
Gln (Q)  84	  3.8%
Glu (E) 138	  6.2%
Gly (G)  87	  3.9%
His (H)  42	  1.9%
Ile (I) 146	  6.5%
Leu (L) 211	  9.5%
Lys (K) 188	  8.4%
Met (M)  42	  1.9%
Phe (F) 102	  4.6%
Pro (P) 108	  4.8%
Ser (S) 210	  9.4%
Thr (T) 110	  4.9%
Trp (W)  19	  0.9%
Tyr (Y)  63	  2.8%
Val (V) 128	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 271
Total number of positively charged residues (Arg + Lys): 298

Atomic composition:

Carbon      C	     11227
Hydrogen    H	     17839
Nitrogen    N	      3081
Oxygen      O	      3386
Sulfur      S	        73

Formula: C11227H17839N3081O3386S73
Total number of atoms: 35606

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   200245
Abs 0.1% (=1 g/l)   0.793, assuming all pairs of Cys residues form cystines


Ext. coefficient   198370
Abs 0.1% (=1 g/l)   0.786, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.89
This classifies the protein as unstable.



Aliphatic index: 85.05

Grand average of hydropathicity (GRAVY): -0.441