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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSNRCQTNE VNKFISSTEK GPFTGRDNTL SFNKIGSRLN SPPILKDKIE LKFLQHSEDL

70 80 90 100 110 120
NQSRSYVNIR PRTLEDQSYK FEAPNLNDNE TSWAKDFRYN FPKNVEPPIE NQIANLNINN

130 140 150 160 170 180
GLRTSQTDFP LGFYSQKNFN IASFPVVDHQ IFKTTGLEHP INSHIDSLIN AEFSELEASS

190 200 210 220 230 240
LEEDVHTEEE NSGTSLEDEE TAMKGLASDI IEFCDNNSAN KDVKERLNSS KFMGLMGSIS

250 260 270 280
DGSIVLKKDN GTERNLQKHV GFCFQNSGNW AGLEFHDVED RIA


References and documentation are available.

Number of amino acids: 283 Molecular weight: 32037.29 Theoretical pI: 4.93
Amino acid composition: 
Ala (A) 11 3.9% Arg (R) 11 3.9% Asn (N) 32 11.3% Asp (D) 18 6.4% Cys (C) 3 1.1% Gln (Q) 10 3.5% Glu (E) 26 9.2% Gly (G) 15 5.3% His (H) 7 2.5% Ile (I) 18 6.4% Leu (L) 22 7.8% Lys (K) 18 6.4% Met (M) 4 1.4% Phe (F) 18 6.4% Pro (P) 11 3.9% Ser (S) 29 10.2% Thr (T) 14 4.9% Trp (W) 2 0.7% Tyr (Y) 4 1.4% Val (V) 10 3.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 44 Total number of positively charged residues (Arg + Lys): 29 Atomic composition: Carbon C 1396 Hydrogen H 2162 Nitrogen N 392 Oxygen O 461 Sulfur S 7 Formula: C1396H2162N392O461S7 Total number of atoms: 4418 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17085 Abs 0.1% (=1 g/l) 0.533, assuming all pairs of Cys residues form cystines Ext. coefficient 16960 Abs 0.1% (=1 g/l) 0.529, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.34 This classifies the protein as unstable. Aliphatic index: 69.26 Grand average of hydropathicity (GRAVY): -0.759