ProtParam
User-provided sequence:
10 20 30 40 50 60
MNSNRCQTNE VNKFISSTEK GPFTGRDNTL SFNKIGSRLN SPPILKDKIE LKFLQHSEDL
70 80 90 100 110 120
NQSRSYVNIR PRTLEDQSYK FEAPNLNDNE TSWAKDFRYN FPKNVEPPIE NQIANLNINN
130 140 150 160 170 180
GLRTSQTDFP LGFYSQKNFN IASFPVVDHQ IFKTTGLEHP INSHIDSLIN AEFSELEASS
190 200 210 220 230 240
LEEDVHTEEE NSGTSLEDEE TAMKGLASDI IEFCDNNSAN KDVKERLNSS KFMGLMGSIS
250 260 270 280
DGSIVLKKDN GTERNLQKHV GFCFQNSGNW AGLEFHDVED RIA
References and
documentation are available.
Number of amino acids: 283
Molecular weight: 32037.29
Theoretical pI: 4.93
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1396
Hydrogen H 2162
Nitrogen N 392
Oxygen O 461
Sulfur S 7
Formula: C
1396H
2162N
392O
461S
7
Total number of atoms: 4418
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17085
Abs 0.1% (=1 g/l) 0.533, assuming all pairs of Cys residues form cystines
Ext. coefficient 16960
Abs 0.1% (=1 g/l) 0.529, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.34
This classifies the protein as unstable.
Aliphatic index: 69.26
Grand average of hydropathicity (GRAVY): -0.759