ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSQGYDESS SSTAATSGPT SGDPRMGKKQ RFMNLIRTTK DVYIPNLTSS ISQKTMDGIR 

        70         80         90        100        110        120 
STTNSFEGYN DLPMELPHNT TITYFPTYTT TNLVDPDGLS APRKDFETTV RCAVSYPGNP 

       130        140        150        160        170        180 
TSRRNRWLLS LCKQYLRTGT AEADVAPVVP PHLEEDSGDL NDSQSSIESS LSSKSENRYS 

       190        200        210        220        230        240 
HMGIQEEDVL NERIQGFLSK KVPNTPVVVD LLPKDKLRGD TASFFGTTDS YGNLLIKAET 

       250        260        270        280        290        300 
DFLPSKINIT LDTPIEGHAD PISETFPANY VSPYGIGLIS DIDDTIKHTG VTGDRRSMFR 

       310        320        330        340        350        360 
NVFIHDVQSW VIDGVPLWYK TLHDVADVDF FYVSNSPIQT FTLLKQYICA NFPPGPIFLK 

       370        380        390        400        410        420 
QYSGNFFSTI MTSSANRKIQ PIANILKDFP KKKFILVGDS GEHDLEAYTT TALQFPNQIL 

       430        440        450        460        470        480 
AIYIRCCSNS MSDVPSHDEE VMNEVNNIIE LQQRPMQMTK STVRTRRRPP PPPIPSTQKP 

       490        500        510        520        530        540 
SLTEEQTESI RMSRRNKDEN NAKRVAPPPL PNRQLPNLDA NTYYVPSSQN DYGMYGAFMD 

       550        560        570        580 
KKADEWKRRV MDSIQKLSNQ DTTLMFFSDP ALSLEDSIRR IREKYSN 

References and documentation are available.

Number of amino acids: 587

Molecular weight: 66134.33

Theoretical pI: 5.83

Amino acid composition: 
Ala (A)  24	  4.1%
Arg (R)  33	  5.6%
Asn (N)  36	  6.1%
Asp (D)  43	  7.3%
Cys (C)   5	  0.9%
Gln (Q)  23	  3.9%
Glu (E)  28	  4.8%
Gly (G)  27	  4.6%
His (H)   9	  1.5%
Ile (I)  37	  6.3%
Leu (L)  42	  7.2%
Lys (K)  31	  5.3%
Met (M)  17	  2.9%
Phe (F)  24	  4.1%
Pro (P)  45	  7.7%
Ser (S)  59	 10.1%
Thr (T)  49	  8.3%
Trp (W)   4	  0.7%
Tyr (Y)  22	  3.7%
Val (V)  29	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2910
Hydrogen    H	      4560
Nitrogen    N	       798
Oxygen      O	       919
Sulfur      S	        22

Formula: C2910H4560N798O919S22
Total number of atoms: 9209

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55030
Abs 0.1% (=1 g/l)   0.832, assuming all pairs of Cys residues form cystines


Ext. coefficient    54780
Abs 0.1% (=1 g/l)   0.828, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.27
This classifies the protein as unstable.



Aliphatic index: 70.90

Grand average of hydropathicity (GRAVY): -0.590