ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSRILQVFQ RELTCPICMN YFIDPVTIDC GHSFCRPCFY LIWQDITVLP QCSKCKKTTW 

        70         80         90        100        110        120 
QRNLRTNIRL KNMASIARKA SLRQFLSSEE QICGTHREIK KMFCEVDKSL LCLLCSNSQE 

       130        140        150        160        170        180 
HRDHKHCPIE WAAEEHREKL LKKMLSLWEK ACENHRNLNM ETTRTRCWKD YVSLRREAIR 

       190        200        210        220        230        240 
AEYQKMPAFL HDEEQHHLER LQKEGEDIFQ QLNESKARME HSRELLRGMY EDLKEMCHKA 

       250        260        270        280        290        300 
DVELLQAFGD ILHRYESLLL QVPEPANPEL SAGPITGLLD RLNGFRVDFT LQPERANSHI 

       310        320        330        340        350        360 
FLYGDLRSMN VGCDPQDDPH ITAKSECFLV WGAKTFTSGK YYWEVHVGDS WNWAFGVCNN 

       370        380        390        400        410        420 
YWKEKTQNDK IDGGEGLFLL GCVKEDAHSS LFTTSPLVVQ YVPRPTSRVG LFLDCEGRTM 

       430        440        450 
SFVDVDQNAL IYTIPNCSFS PPLRPVFCCS HF 

References and documentation are available.

Number of amino acids: 452

Molecular weight: 52694.36

Theoretical pI: 6.53

Amino acid composition: 
Ala (A)  20	  4.4%
Arg (R)  30	  6.6%
Asn (N)  20	  4.4%
Asp (D)  24	  5.3%
Cys (C)  23	  5.1%
Gln (Q)  20	  4.4%
Glu (E)  36	  8.0%
Gly (G)  20	  4.4%
His (H)  18	  4.0%
Ile (I)  20	  4.4%
Leu (L)  48	 10.6%
Lys (K)  26	  5.8%
Met (M)  12	  2.7%
Phe (F)  22	  4.9%
Pro (P)  20	  4.4%
Ser (S)  29	  6.4%
Thr (T)  21	  4.6%
Trp (W)  10	  2.2%
Tyr (Y)  12	  2.7%
Val (V)  21	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2329
Hydrogen    H	      3610
Nitrogen    N	       654
Oxygen      O	       675
Sulfur      S	        35

Formula: C2329H3610N654O675S35
Total number of atoms: 7303

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74255
Abs 0.1% (=1 g/l)   1.409, assuming all pairs of Cys residues form cystines


Ext. coefficient    72880
Abs 0.1% (=1 g/l)   1.383, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.76
This classifies the protein as unstable.



Aliphatic index: 76.57

Grand average of hydropathicity (GRAVY): -0.460