ProtParam
User-provided sequence:
10 20 30 40 50 60
MNSSNDLSDE LIRRLSVGGA LEEVIASEMF EDSIDVETEA EPDDIIIVHM DIREPLSMLK
70 80 90 100 110 120
SLVEQKIGVC LNYYTFWLQD AQELESHKNL VDQCVKGEGL VQINVQIQTI RKRINIADVL
130 140 150 160 170 180
KPTEAALAAL AEEVVGQLSP PETASQKSSS SESPIKTPLK RMHKEDSEEE SVEGKDVKPV
190 200 210 220 230 240
LNWVLDSKFK REQIRLKIPE AANEWTHAHV TYWLEWAVKQ FELVGINMSD WQMNGQELCA
250 260 270 280 290 300
MTHEEFNQKL PRDPGNIFWT HLQLLKECNF VSVVHKRAEE QRKPKQPRIM SANSISTNSG
310 320 330 340 350 360
GSLSLEQRIM RKSYQSVKSS DSVESTTSSM NPSNYTTIGS GNNGQVQLWQ FLLEILTDCE
370 380 390 400 410 420
HTDVIEWVGT EGEFKLTDPD RVARLWGEKK NKPAMNYEKL SRALRYYYDG DMISKVSGKR
430 440 450 460
FAYKFDCDLK LLIGYDANEL STLVSEGKTA PERVAATETI TEDT
References and
documentation are available.
Number of amino acids: 464
Molecular weight: 52644.46
Theoretical pI: 5.01
Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 2312
Hydrogen H 3667
Nitrogen N 629
Oxygen O 735
Sulfur S 19
Formula: C
2312H
3667N
629O
735S
19
Total number of atoms: 7362
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 71765
Abs 0.1% (=1 g/l) 1.363, assuming all pairs of Cys residues form cystines
Ext. coefficient 71390
Abs 0.1% (=1 g/l) 1.356, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.10
This classifies the protein as unstable.
Aliphatic index: 83.60
Grand average of hydropathicity (GRAVY): -0.513