ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNTDQQPYQG QTDYTQGPGN GQSQEQDYDQ YGQPLYPSQA DGYYDPNVAA GTEADMYGQQ 

        70         80         90        100        110        120 
PPNESYDQDY TNGEYYGQPP NMAAQDGENF SDFSSYGPPG TPGYDSYGGQ YTASQMSYGE 

       130        140        150        160        170        180 
PNSSGTSTPI YGNYDPNAIA MALPNEPYPA WTADSQSPVS IEQIEDIFID LTNRLGFQRD 

       190        200        210        220        230        240 
SMRNMFDHFM VLLDSRSSRM SPDQALLSLH ADYIGGDTAN YKKWYFAAQL DMDDEIGFRN 

       250        260        270        280        290        300 
MSLGKLSRKA RKAKKKNKKA MEEANPEDTE ETLNKIEGDN SLEAADFRWK AKMNQLSPLE 

       310        320        330        340        350        360 
RVRHIALYLL CWGEANQVRF TAECLCFIYK CALDYLDSPL CQQRQEPMPE GDFLNRVITP 

       370        380        390        400        410        420 
IYHFIRNQVY EIVDGRFVKR ERDHNKIVGY DDLNQLFWYP EGIAKIVLED GTKLIELPLE 

       430        440        450        460        470        480 
ERYLRLGDVV WDDVFFKTYK ETRTWLHLVT NFNRIWVMHI SIFWMYFAYN SPTFYTHNYQ 

       490        500        510        520        530        540 
QLVDNQPLAA YKWASCALGG TVASLIQIVA TLCEWSFVPR KWAGAQHLSR RFWFLCIIFG 

       550        560        570        580        590        600 
INLGPIIFVF AYDKDTVYST AAHVVAAVMF FVAVATIIFF SIMPLGGLFT SYMKKSTRRY 

       610        620        630        640        650        660 
VASQTFTAAF APLHGLDRWM SYLVWVTVFA AKYSESYYFL VLSLRDPIRI LSTTAMRCTG 

       670        680        690        700        710        720 
EYWWGAVLCK VQPKIVLGLV IATDFILFFL DTYLWYIIVN TIFSVGKSFY LGISILTPWR 

       730        740        750        760        770        780 
NIFTRLPKRI YSKILATTDM EIKYKPKVLI SQVWNAIIIS MYREHLLAID HVQKLLYHQV 

       790        800        810        820        830        840 
PSEIEGKRTL RAPTFFVSQD DNNFETEFFP RDSEAERRIS FFAQSLSTPI PEPLPVDNMP 

       850        860        870        880        890        900 
TFTVLTPHYA ERILLSLREI IREDDQFSRV TLLEYLKQLH PVEWECFVKD TKILAEETAA 

       910        920        930        940        950        960 
YEGNENEAEK EDALKSQIDD LPFYCIGFKS AAPEYTLRTR IWASLRSQTL YRTISGFMNY 

       970        980        990       1000       1010       1020 
SRAIKLLYRV ENPEIVQMFG GNAEGLEREL EKMARRKFKF LVSMQRLAKF KPHELENAEF 

      1030       1040       1050       1060       1070       1080 
LLRAYPDLQI AYLDEEPPLT EGEEPRIYSA LIDGHCEILD NGRRRPKFRV QLSGNPILGD 

      1090       1100       1110       1120       1130       1140 
GKSDNQNHAL IFYRGEYIQL IDANQDNYLE ECLKIRSVLA EFEELNVEQV NPYAPGLRYE 

      1150       1160       1170       1180       1190       1200 
EQTTNHPVAI VGAREYIFSE NSGVLGDVAA GKEQTFGTLF ARTLSQIGGK LHYGHPDFIN 

      1210       1220       1230       1240       1250       1260 
ATFMTTRGGV SKAQKGLHLN EDIYAGMNAM LRGGRIKHCE YYQCGKGRDL GFGTILNFTT 

      1270       1280       1290       1300       1310       1320 
KIGAGMGEQM LSREYYYLGT QLPVDRFLTF YYAHPGFHLN NLFIQLSLQM FMLTLVNLSS 

      1330       1340       1350       1360       1370       1380 
LAHESIMCIY DRNKPKTDVL VPIGCYNFQP AVDWVRRYTL SIFIVFWIAF VPIVVQELIE 

      1390       1400       1410       1420       1430       1440 
RGLWKATQRF FCHLLSLSPM FEVFAGQIYS SALLSDLAIG GARYISTGRG FATSRIPFSI 

      1450       1460       1470       1480       1490       1500 
LYSRFAGSAI YMGARSMLML LFGTVAHWQA PLLWFWASLS SLIFAPFVFN PHQFAWEDFF 

      1510       1520       1530       1540       1550       1560 
LDYRDYIRWL SRGNNQYHRN SWIGYVRMSR ARITGFKRKL VGDESEKAAG DASRAHRTNL 

      1570       1580       1590       1600       1610       1620 
IMAEIIPCAI YAAGCFIAFT FINAQTGVKT TDDDRVNSVL RIIICTLAPI AVNLGVLFFC 

      1630       1640       1650       1660       1670       1680 
MGMSCCSGPL FGMCCKKTGS VMAGIAHGVA VIVHIAFFIV MWVLESFNFV RMLIGVVTCI 

      1690       1700       1710       1720       1730       1740 
QCQRLIFHCM TALMLTREFK NDHANTAFWT GKWYGKGMGY MAWTQPSREL TAKVIELSEF 

      1750       1760       1770       1780       1790       1800 
AADFVLGHVI LICQLPLIII PKIDKFHSIM LFWLKPSRQI RPPIYSLKQT RLRKRMVKKY 

      1810       1820       1830       1840       1850       1860 
CSLYFLVLAI FAGCIIGPAV ASAKIHKHIG DSLDGVVHNL FQPINTTNND TGSQMSTYQS 

      1870 
HYYTHTPSLK TWSTIK 

References and documentation are available.

Number of amino acids: 1876

Molecular weight: 214850.95

Theoretical pI: 6.84

Amino acid composition: 
Ala (A) 138	  7.4%
Arg (R) 102	  5.4%
Asn (N)  77	  4.1%
Asp (D)  89	  4.7%
Cys (C)  33	  1.8%
Gln (Q)  80	  4.3%
Glu (E) 100	  5.3%
Gly (G) 118	  6.3%
His (H)  43	  2.3%
Ile (I) 134	  7.1%
Leu (L) 175	  9.3%
Lys (K)  82	  4.4%
Met (M)  55	  2.9%
Phe (F) 115	  6.1%
Pro (P)  86	  4.6%
Ser (S) 114	  6.1%
Thr (T) 102	  5.4%
Trp (W)  37	  2.0%
Tyr (Y)  99	  5.3%
Val (V)  97	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 189
Total number of positively charged residues (Arg + Lys): 184

Atomic composition:

Carbon      C	      9802
Hydrogen    H	     14916
Nitrogen    N	      2544
Oxygen      O	      2727
Sulfur      S	        88

Formula: C9802H14916N2544O2727S88
Total number of atoms: 30077

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   353010
Abs 0.1% (=1 g/l)   1.643, assuming all pairs of Cys residues form cystines


Ext. coefficient   351010
Abs 0.1% (=1 g/l)   1.634, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.44
This classifies the protein as unstable.



Aliphatic index: 86.59

Grand average of hydropathicity (GRAVY): -0.109