ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNTFFKLGSL IQRTASQISS SFPKSRFFSD GESAVYHHAR LFKKPLSTKL KFNLVNSVSL 

        70         80         90        100        110        120 
MGFVDRSIQV MNTGPDRFGV FTILRVKDPL NPNRSFRISL RMWDAMARTC IAHLKLNDHI 

       130        140        150        160        170        180 
LVSGRLESYS KSSSDVYSGL NLDYQVKVAE VNYVAAPPSH VLDSQISKNP KTKTEDDIEE 

       190        200        210        220        230        240 
SKKDEIYLWQ VFFSNPYDWW DNRRNKKNPK QPDFKHKDTG EALWLCSDLP DWITRRLELF 

       250        260 
DQKNRFYDEE KTRRDRLSDY I 

References and documentation are available.

Number of amino acids: 261

Molecular weight: 30563.68

Theoretical pI: 9.34

Amino acid composition: 
Ala (A)  10	  3.8%
Arg (R)  19	  7.3%
Asn (N)  15	  5.7%
Asp (D)  22	  8.4%
Cys (C)   2	  0.8%
Gln (Q)   8	  3.1%
Glu (E)  11	  4.2%
Gly (G)   8	  3.1%
His (H)   6	  2.3%
Ile (I)  12	  4.6%
Leu (L)  24	  9.2%
Lys (K)  22	  8.4%
Met (M)   5	  1.9%
Phe (F)  16	  6.1%
Pro (P)  12	  4.6%
Ser (S)  28	 10.7%
Thr (T)  11	  4.2%
Trp (W)   6	  2.3%
Tyr (Y)   9	  3.4%
Val (V)  15	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1372
Hydrogen    H	      2123
Nitrogen    N	       381
Oxygen      O	       399
Sulfur      S	         7

Formula: C1372H2123N381O399S7
Total number of atoms: 4282

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46535
Abs 0.1% (=1 g/l)   1.523, assuming all pairs of Cys residues form cystines


Ext. coefficient    46410
Abs 0.1% (=1 g/l)   1.518, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.47
This classifies the protein as unstable.



Aliphatic index: 74.29

Grand average of hydropathicity (GRAVY): -0.654