ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNTFFLSLLS GAAAGTSTDL VFFPIDTIKT RLQAKGGFFA NGGYKGIYRG LGSAVVASAP 

        70         80         90        100        110        120 
GASLFFISYD YMKVKSRPYI SKLYSQGSEQ LIDTTTHMLS SSIGEICACL VRVPAEVVKQ 

       130        140        150        160        170        180 
RTQVHSTNSS WQTLQSILRN DNKEGLRKNL YRGWSTTIMR EIPFTCIQFP LYEYLKKTWA 

       190        200        210        220        230        240 
KANGQSQVEP WKGAICGSIA GGIAAATTTP LDFLKTRLML NKTTASLGSV IIRIYREEGP 

       250        260        270        280 
AVFFSGVGPR TMWISAGGAI FLGMYETVHS LLSKSFPTAG EMRA 

References and documentation are available.

Number of amino acids: 284

Molecular weight: 31026.85

Theoretical pI: 9.67

Amino acid composition: 
Ala (A)  24	  8.5%
Arg (R)  14	  4.9%
Asn (N)   8	  2.8%
Asp (D)   6	  2.1%
Cys (C)   4	  1.4%
Gln (Q)  10	  3.5%
Glu (E)  11	  3.9%
Gly (G)  27	  9.5%
His (H)   3	  1.1%
Ile (I)  20	  7.0%
Leu (L)  25	  8.8%
Lys (K)  16	  5.6%
Met (M)   8	  2.8%
Phe (F)  15	  5.3%
Pro (P)  11	  3.9%
Ser (S)  28	  9.9%
Thr (T)  24	  8.5%
Trp (W)   5	  1.8%
Tyr (Y)  11	  3.9%
Val (V)  14	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1401
Hydrogen    H	      2201
Nitrogen    N	       371
Oxygen      O	       400
Sulfur      S	        12

Formula: C1401H2201N371O400S12
Total number of atoms: 4385

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44140
Abs 0.1% (=1 g/l)   1.423, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   1.415, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.02
This classifies the protein as stable.



Aliphatic index: 84.54

Grand average of hydropathicity (GRAVY): 0.036