ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA RSLSTIPSYE 

        70         80         90        100        110        120 
QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD YIPTIFENYV TNIEGPNGQI 

       130        140        150        160        170        180 
IELALWDTAG QEEYSRLRPL SYTNADVLMV CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI 

       190        200        210        220        230        240 
MLVGLKSDLY EADNLSDLVE PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS 

       250        260        270        280        290 
DSLYAPREPT HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M 

References and documentation are available.

Number of amino acids: 291

Molecular weight: 32185.56

Theoretical pI: 6.24

Amino acid composition: 
Ala (A)  13	  4.5%
Arg (R)  13	  4.5%
Asn (N)  17	  5.8%
Asp (D)  15	  5.2%
Cys (C)   6	  2.1%
Gln (Q)   6	  2.1%
Glu (E)  18	  6.2%
Gly (G)  17	  5.8%
His (H)   5	  1.7%
Ile (I)  18	  6.2%
Leu (L)  30	 10.3%
Lys (K)  18	  6.2%
Met (M)   5	  1.7%
Phe (F)   9	  3.1%
Pro (P)  19	  6.5%
Ser (S)  31	 10.7%
Thr (T)  23	  7.9%
Trp (W)   2	  0.7%
Tyr (Y)  10	  3.4%
Val (V)  16	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1421
Hydrogen    H	      2263
Nitrogen    N	       383
Oxygen      O	       445
Sulfur      S	        11

Formula: C1421H2263N383O445S11
Total number of atoms: 4523

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26275
Abs 0.1% (=1 g/l)   0.816, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.805, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.86
This classifies the protein as unstable.



Aliphatic index: 84.74

Grand average of hydropathicity (GRAVY): -0.338