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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL MKNTLPPSAK

70 80 90 100 110 120
VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED ILISLHALGF ENYAEVLKIY

130 140
LAKYRQQQAL KNQLMYEQDD EEVP


References and documentation are available.

Number of amino acids: 144 Molecular weight: 16153.97 Theoretical pI: 4.78
Amino acid composition: 
Ala (A) 11 7.6% Arg (R) 6 4.2% Asn (N) 10 6.9% Asp (D) 8 5.6% Cys (C) 3 2.1% Gln (Q) 10 6.9% Glu (E) 15 10.4% Gly (G) 5 3.5% His (H) 2 1.4% Ile (I) 7 4.9% Leu (L) 13 9.0% Lys (K) 9 6.2% Met (M) 4 2.8% Phe (F) 2 1.4% Pro (P) 5 3.5% Ser (S) 15 10.4% Thr (T) 7 4.9% Trp (W) 1 0.7% Tyr (Y) 4 2.8% Val (V) 7 4.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 23 Total number of positively charged residues (Arg + Lys): 15 Atomic composition: Carbon C 689 Hydrogen H 1108 Nitrogen N 196 Oxygen O 237 Sulfur S 7 Formula: C689H1108N196O237S7 Total number of atoms: 2237 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 11585 Abs 0.1% (=1 g/l) 0.717, assuming all pairs of Cys residues form cystines Ext. coefficient 11460 Abs 0.1% (=1 g/l) 0.709, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 55.81 This classifies the protein as unstable. Aliphatic index: 75.90 Grand average of hydropathicity (GRAVY): -0.703