ProtParam
User-provided sequence:
10 20 30 40 50 60
MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL MKNTLPPSAK
70 80 90 100 110 120
VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED ILISLHALGF ENYAEVLKIY
130 140
LAKYRQQQAL KNQLMYEQDD EEVP
References and
documentation are available.
Number of amino acids: 144
Molecular weight: 16153.97
Theoretical pI: 4.78
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 689
Hydrogen H 1108
Nitrogen N 196
Oxygen O 237
Sulfur S 7
Formula: C
689H
1108N
196O
237S
7
Total number of atoms: 2237
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.717, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.709, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.81
This classifies the protein as unstable.
Aliphatic index: 75.90
Grand average of hydropathicity (GRAVY): -0.703