ProtParam
User-provided sequence:
10 20 30 40 50 60
MNTNPVTNGF VKPVPTEEEQ AKIEEVRKLL GPLPEKLSSF CSDDAVLRYL RARNWHVKKA
70 80 90 100 110 120
TKMLKETLKW RVQYKPEEIC WEEVAGEAET GKIYRSSCVD KLGRPVLIMR PSVENSKSVK
130 140 150 160 170 180
GQIRYLVYCM ENAVQNLPPG EEQMVWMIDF HGYSLANVSL RTTKETAHVL QEHYPERLAF
190 200 210 220 230 240
AVLYNPPKFF EPFWKVARPF LEPKTRNKVK FVYSDDPNTK VIMEENFDME KMELAFGGND
250 260 270 280 290 300
DSGFNIEKHS ERMKEDDKKR LASLEGIVSA SLDSLSILSV SDGTASNSAH PSSHDVSEDE
H
References and
documentation are available.
Number of amino acids: 301
Molecular weight: 34433.17
Theoretical pI: 5.85
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1529
Hydrogen H 2394
Nitrogen N 414
Oxygen O 463
Sulfur S 14
Formula: C
1529H
2394N
414O
463S
14
Total number of atoms: 4814
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41160
Abs 0.1% (=1 g/l) 1.195, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 1.188, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.69
This classifies the protein as unstable.
Aliphatic index: 73.79
Grand average of hydropathicity (GRAVY): -0.587