ProtParam
User-provided sequence:
10 20 30 40 50 60
MNVARRRSGL FRSTIGATPK ITRGRAAAPS TKEANSTTIP RQSAPGGITI GAAARRPPSR
70 80 90 100 110 120
LPTPTTPATG RASLPERSAM AKPASCSRPI PTMQSTASRI STLTAASTFR QLRTGRPPPP
130 140 150 160 170 180
STQRSTATSS LKPSVTRARP VAQKPILPSK MALLEEKIAQ LESAMAEATD FAEHQKSKIQ
190 200 210 220 230 240
ILDGKLEGAD RKLISLQDQL STLKEVNKAK VEECEDYRVH NNDLRDLLNE KEAELRKLHN
250 260 270 280 290 300
DVVDLRGQIR VAVRVRPLIK SEADASSSAI EYPAIDTIRI NEGSKPGIVV KFEKVFTPVF
310 320 330 340 350 360
SQKEVFANVE EFIRSSLHGY NVGLIAYGQT GSGKTHTMRG GNGEEEGIIP RAAAFLFAES
370 380 390 400 410 420
RKLESLGWKF DFSLSFLEVY NNVAYDLLSD RAVVQLRLND QTVSMIGLSE HTISNVSDVA
430 440 450 460 470 480
RLLRVADGGR KTAATKCNES SSRSHAVYMW KITAHQPSTG ISTSCQLKLV DLAGSERAKE
490 500 510 520 530 540
SGVSGDQFKE MTNINQSLSI LQMCISQQRS QKGHVSYRDS KLTQVLMDCL GRGNSKTMVV
550 560 570 580
VNLNPCNEQA TESKRSIEFA SKMRSTNIGS AVQQRTLLGD VSQMSMN
References and
documentation are available.
Number of amino acids: 587
Molecular weight: 64072.94
Theoretical pI: 9.76
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 81
Atomic composition:
Carbon C 2763
Hydrogen H 4556
Nitrogen N 832
Oxygen O 871
Sulfur S 22
Formula: C
2763H
4556N
832O
871S
22
Total number of atoms: 9044
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23295
Abs 0.1% (=1 g/l) 0.364, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.358, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.63
This classifies the protein as unstable.
Aliphatic index: 80.14
Grand average of hydropathicity (GRAVY): -0.421