ProtParam
User-provided sequence:
10 20 30 40 50 60
MNVIAFSCRK DSGQEPSQSR WRPYLKYIII VAVFLIVVAI ATVTIVLIAN RVDGSANTST
70 80 90 100 110 120
VPNVSSTVAK STITTRAPPV CNKRIIGYYF ATQTSVITRD QVSKLTHAVF AFVNMTSDGH
130 140 150 160 170 180
LQIDGDLAKN RFTNLIEIAK QQTPQVKVMI SIGGNDNSNN FKPGLSSPDR KKLFINSTVS
190 200 210 220 230 240
FLQTYDIDGV DLYWKWPGKT SKDIYSQFIN DLRYSLQRQK RNYIMSIVLP PPDMGANYEA
250 260 270 280 290 300
GIDIENIFDN VDFLNIFTMG YFGPWQNDAG MITGAASQLF NGVNGVPGRK TYNIHHSTER
310 320 330 340 350 360
YVCKTGQSDK YNIAIPFYTM LWKHVKGPVN PPNIEIYRNA TFQGGVVGET SMSRKLVQEE
370 380 390 400 410 420
GYDFSNPTYN PEIRAAFKYN ATTETFLTFE TNDTIAAKID YVKDRILGGV WIWAVDMDDD
430 440
SNNLLNLVKF TGYCTVGNLT LYNC
References and
documentation are available.
Number of amino acids: 444
Molecular weight: 49829.68
Theoretical pI: 8.57
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 2243
Hydrogen H 3467
Nitrogen N 593
Oxygen O 663
Sulfur S 15
Formula: C
2243H
3467N
593O
663S
15
Total number of atoms: 6981
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 71530
Abs 0.1% (=1 g/l) 1.435, assuming all pairs of Cys residues form cystines
Ext. coefficient 71280
Abs 0.1% (=1 g/l) 1.430, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.03
This classifies the protein as stable.
Aliphatic index: 84.05
Grand average of hydropathicity (GRAVY): -0.204