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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNVIAFSCRK DSGQEPSQSR WRPYLKYIII VAVFLIVVAI ATVTIVLIAN RVDGSANTST

70 80 90 100 110 120
VPNVSSTVAK STITTRAPPV CNKRIIGYYF ATQTSVITRD QVSKLTHAVF AFVNMTSDGH

130 140 150 160 170 180
LQIDGDLAKN RFTNLIEIAK QQTPQVKVMI SIGGNDNSNN FKPGLSSPDR KKLFINSTVS

190 200 210 220 230 240
FLQTYDIDGV DLYWKWPGKT SKDIYSQFIN DLRYSLQRQK RNYIMSIVLP PPDMGANYEA

250 260 270 280 290 300
GIDIENIFDN VDFLNIFTMG YFGPWQNDAG MITGAASQLF NGVNGVPGRK TYNIHHSTER

310 320 330 340 350 360
YVCKTGQSDK YNIAIPFYTM LWKHVKGPVN PPNIEIYRNA TFQGGVVGET SMSRKLVQEE

370 380 390 400 410 420
GYDFSNPTYN PEIRAAFKYN ATTETFLTFE TNDTIAAKID YVKDRILGGV WIWAVDMDDD

430 440
SNNLLNLVKF TGYCTVGNLT LYNC


References and documentation are available.

Number of amino acids: 444 Molecular weight: 49829.68 Theoretical pI: 8.57
Amino acid composition: 
Ala (A) 26 5.9% Arg (R) 18 4.1% Asn (N) 36 8.1% Asp (D) 27 6.1% Cys (C) 5 1.1% Gln (Q) 17 3.8% Glu (E) 12 2.7% Gly (G) 29 6.5% His (H) 5 1.1% Ile (I) 38 8.6% Leu (L) 25 5.6% Lys (K) 25 5.6% Met (M) 10 2.3% Phe (F) 22 5.0% Pro (P) 20 4.5% Ser (S) 29 6.5% Thr (T) 36 8.1% Trp (W) 7 1.6% Tyr (Y) 22 5.0% Val (V) 35 7.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 43 Atomic composition: Carbon C 2243 Hydrogen H 3467 Nitrogen N 593 Oxygen O 663 Sulfur S 15 Formula: C2243H3467N593O663S15 Total number of atoms: 6981 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 71530 Abs 0.1% (=1 g/l) 1.435, assuming all pairs of Cys residues form cystines Ext. coefficient 71280 Abs 0.1% (=1 g/l) 1.430, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.03 This classifies the protein as stable. Aliphatic index: 84.05 Grand average of hydropathicity (GRAVY): -0.204