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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNVSFSTSSL SVSNSRTRLI SFRETEHGDQ KSSEEWKTST KLEALGSMEG ELAHLLSTCK

70 80 90 100 110 120
PETLNHNKTE LSGFRNLFAR FLRAKPTVDW SKIEPLPEGA IRPYKSLGHV VDKELIASQL

130 140 150 160 170 180
RKLVVVKLNG GLGTSMGCKG PKSVISVRND LTFLDLTMQQ IQTLNKTYGV DVPLVLMNSF

190 200 210 220 230 240
NTNEDTQKVL KKYANVKVSV HTFSQSQYPR INRETLLPIV KSLEADDNEC WYPPGHGNFY

250 260 270 280 290 300
EAFHNSGLLD KFLADGKEYC FLSNIDNMGA TVDLSILNFV LNPSPDQTAP EFLMEVTDKT

310 320 330 340 350 360
RADVKGGTLI QYEGRLMLLE IAQVPKDYVD EFKSISKFRI FNTNNLWAKL DAMRKIVVEN

370 380 390 400 410 420
KLEMEVIVNP KHLDRGLDVI QLETAAGAAI KSFSGAVGVN VPRSRFLPVK KTSDLLLLMS

430 440 450 460 470 480
NLYDIDNGSL TLSHQRSFPT TPLVKLGSSF DKVKDYLGRF NGIPDLLELD HLTVSGDVWF

490 500 510 520
GKDVSLKGTV IIIANHGDRI DIPPGSILEN KIVSGNLRIL EH


References and documentation are available.

Number of amino acids: 522 Molecular weight: 58217.77 Theoretical pI: 6.88
Amino acid composition: 
Ala (A) 22 4.2% Arg (R) 22 4.2% Asn (N) 32 6.1% Asp (D) 32 6.1% Cys (C) 4 0.8% Gln (Q) 13 2.5% Glu (E) 30 5.7% Gly (G) 34 6.5% His (H) 12 2.3% Ile (I) 28 5.4% Leu (L) 65 12.5% Lys (K) 39 7.5% Met (M) 11 2.1% Phe (F) 23 4.4% Pro (P) 23 4.4% Ser (S) 45 8.6% Thr (T) 31 5.9% Trp (W) 5 1.0% Tyr (Y) 11 2.1% Val (V) 40 7.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 62 Total number of positively charged residues (Arg + Lys): 61 Atomic composition: Carbon C 2603 Hydrogen H 4157 Nitrogen N 701 Oxygen O 779 Sulfur S 15 Formula: C2603H4157N701O779S15 Total number of atoms: 8255 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 44140 Abs 0.1% (=1 g/l) 0.758, assuming all pairs of Cys residues form cystines Ext. coefficient 43890 Abs 0.1% (=1 g/l) 0.754, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 21.67 This classifies the protein as stable. Aliphatic index: 95.92 Grand average of hydropathicity (GRAVY): -0.220