ProtParam
User-provided sequence:
10 20 30 40 50 60
MNVSFSTSSL SVSNSRTRLI SFRETEHGDQ KSSEEWKTST KLEALGSMEG ELAHLLSTCK
70 80 90 100 110 120
PETLNHNKTE LSGFRNLFAR FLRAKPTVDW SKIEPLPEGA IRPYKSLGHV VDKELIASQL
130 140 150 160 170 180
RKLVVVKLNG GLGTSMGCKG PKSVISVRND LTFLDLTMQQ IQTLNKTYGV DVPLVLMNSF
190 200 210 220 230 240
NTNEDTQKVL KKYANVKVSV HTFSQSQYPR INRETLLPIV KSLEADDNEC WYPPGHGNFY
250 260 270 280 290 300
EAFHNSGLLD KFLADGKEYC FLSNIDNMGA TVDLSILNFV LNPSPDQTAP EFLMEVTDKT
310 320 330 340 350 360
RADVKGGTLI QYEGRLMLLE IAQVPKDYVD EFKSISKFRI FNTNNLWAKL DAMRKIVVEN
370 380 390 400 410 420
KLEMEVIVNP KHLDRGLDVI QLETAAGAAI KSFSGAVGVN VPRSRFLPVK KTSDLLLLMS
430 440 450 460 470 480
NLYDIDNGSL TLSHQRSFPT TPLVKLGSSF DKVKDYLGRF NGIPDLLELD HLTVSGDVWF
490 500 510 520
GKDVSLKGTV IIIANHGDRI DIPPGSILEN KIVSGNLRIL EH
References and
documentation are available.
Number of amino acids: 522
Molecular weight: 58217.77
Theoretical pI: 6.88
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 2603
Hydrogen H 4157
Nitrogen N 701
Oxygen O 779
Sulfur S 15
Formula: C
2603H
4157N
701O
779S
15
Total number of atoms: 8255
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44140
Abs 0.1% (=1 g/l) 0.758, assuming all pairs of Cys residues form cystines
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 0.754, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 21.67
This classifies the protein as stable.
Aliphatic index: 95.92
Grand average of hydropathicity (GRAVY): -0.220