ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK 

        70         80         90        100        110        120 
EALVERRRAF KRSQKKHKKK HLKHENANDE TTAVEDEDDD LDEDDVNADD DDFMHSETRE 

       130        140        150        160        170        180 
KELESIDVLL EQTTGGNNKD LKNTMQYLTN FSRFRDQETV GAVIQLLKST GLHPFEVAQL 

       190        200        210        220 
GSLACDTADE AKTLIPSLNN KISDDELERI LKELSNLETL Y 

References and documentation are available.

Number of amino acids: 221

Molecular weight: 25414.18

Theoretical pI: 4.82

Amino acid composition: 
Ala (A)  14	  6.3%
Arg (R)  13	  5.9%
Asn (N)  14	  6.3%
Asp (D)  19	  8.6%
Cys (C)   1	  0.5%
Gln (Q)  12	  5.4%
Glu (E)  29	 13.1%
Gly (G)   7	  3.2%
His (H)   6	  2.7%
Ile (I)   8	  3.6%
Leu (L)  28	 12.7%
Lys (K)  18	  8.1%
Met (M)   3	  1.4%
Phe (F)   7	  3.2%
Pro (P)   2	  0.9%
Ser (S)  12	  5.4%
Thr (T)  16	  7.2%
Trp (W)   0	  0.0%
Tyr (Y)   2	  0.9%
Val (V)  10	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1090
Hydrogen    H	      1770
Nitrogen    N	       316
Oxygen      O	       374
Sulfur      S	         4

Formula: C1090H1770N316O374S4
Total number of atoms: 3554

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.117, assuming all pairs of Cys residues form cystines


Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.117, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.88
This classifies the protein as unstable.



Aliphatic index: 82.99

Grand average of hydropathicity (GRAVY): -0.900