ProtParam
User-provided sequence:
10 20 30 40 50 60
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE LEKALSCPGQ
70 80 90 100 110 120
PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH HELKPLECCE FPFGSKQKEV
130 140 150 160 170 180
CINPYHYKRV ESPVLPPVLV PRHSEYNPQH SLLAQFRNLG QNEPHMPLNA TFPDSFQQPH
190 200 210 220 230 240
SHPFPQSPNS SYPNSPGSSS STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMAQDGSQ
250 260 270 280 290 300
PMDTNMTNMT APTLPAEINR GDVQAVAYEE PKHWCSIVYY ELNNRVGEAF HASSTSVLVD
310 320 330 340 350 360
GFTDPSNNKN RFCLGLLSNV NRNSTIENTR RHIGKGVHLY YVGGEVYAEC LSDSSIFVQS
370 380 390 400 410 420
RNCNYHHGFH PTTVCKIPSG CSLKIFNNQE FAQLLAQSVN HGFETVYELT KMCTIRMSFV
430 440 450 460
KGWGAEYHRQ DVTSTPCWIE IHPHGPLQWL DKVLTQMGSP HNPISSVS
References and
documentation are available.
Number of amino acids: 468
Molecular weight: 52532.29
Theoretical pI: 6.90
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 2330
Hydrogen H 3555
Nitrogen N 649
Oxygen O 692
Sulfur S 25
Formula: C
2330H
3555N
649O
692S
25
Total number of atoms: 7251
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 68715
Abs 0.1% (=1 g/l) 1.308, assuming all pairs of Cys residues form cystines
Ext. coefficient 67840
Abs 0.1% (=1 g/l) 1.291, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.82
This classifies the protein as unstable.
Aliphatic index: 64.53
Grand average of hydropathicity (GRAVY): -0.575