ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNVTSPKDGN HSFSKKNRFN TNKPRFHKLN EQAQSINLPE DRDSIVSSNT TSIMTDDAFD 

        70         80         90        100        110        120 
YNEGIASRTK NINSDSDRSN DTIKQNNYNK RETGYNPFYN GSGINQRYTQ FRKREFEPTL 

       130        140        150        160        170        180 
AENKAEEYIS DEDNVKIDED NIENELQFTP KIKEASILRS SLLGQRNVLN TRNPKSKESH 

       190        200        210        220        230        240 
IKVKPIINNK SSSQRKSSAA LRKQLGKPLP LPYLNSPNSD STPTLQRKEE VFTDEVLQKK 

       250        260        270        280        290        300 
RELIESKWHR LLFHDKKMVE KKLESLREYE RKRMPPRGTD VSSSEQDNSF KISTPTKSYV 

       310        320        330        340        350        360 
SLEQKPLPNL SAMNNFNDVT DNKEKEETNN NILKFQAQRD PLQILQSEIE MHTKKLDTII 

       370        380        390        400        410        420 
ELLKDDTDSK EKRKVVTNDN AAPEQMVNKG WRKNVMMIYK KSGNIMKKYR EYFLWTICIL 

       430 
ILLYCNIYVY YRF 

References and documentation are available.

Number of amino acids: 433

Molecular weight: 50653.07

Theoretical pI: 9.30

Amino acid composition: 
Ala (A)  12	  2.8%
Arg (R)  26	  6.0%
Asn (N)  44	 10.2%
Asp (D)  26	  6.0%
Cys (C)   2	  0.5%
Gln (Q)  18	  4.2%
Glu (E)  35	  8.1%
Gly (G)  10	  2.3%
His (H)   6	  1.4%
Ile (I)  28	  6.5%
Leu (L)  33	  7.6%
Lys (K)  48	 11.1%
Met (M)  10	  2.3%
Phe (F)  15	  3.5%
Pro (P)  20	  4.6%
Ser (S)  40	  9.2%
Thr (T)  25	  5.8%
Trp (W)   3	  0.7%
Tyr (Y)  16	  3.7%
Val (V)  16	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      2215
Hydrogen    H	      3544
Nitrogen    N	       636
Oxygen      O	       699
Sulfur      S	        12

Formula: C2215H3544N636O699S12
Total number of atoms: 7106

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40465
Abs 0.1% (=1 g/l)   0.799, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   0.796, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.50
This classifies the protein as unstable.



Aliphatic index: 68.43

Grand average of hydropathicity (GRAVY): -1.055