ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNWKSYVFPG GHPPAALTTG LVVFLTAIYL LSFIFALRED LSLAPESLFK LQMSRLSLYP 

        70         80         90        100        110        120 
LIHLSLPHLL FNVLAIWAPL NLFEETHGTV YTGVFLNLSA LFAGILYCLL GKLLYPEALV 

       130        140        150        160        170        180 
AGASGWCFTL FAYYSFKESQ IRPRTRIFRT DYSIPTLYTP LVLLVAIAVV IPGSSFWGHF 

       190        200        210        220        230        240 
FGLCVGYAIG YKESWFNKIT PPGWIITKIE KSLDGLIRLI PWGIKYYRDE DIDRTKDYEP 

       250        260 
LMSTETPLPL HNDNSGTVLG TA 

References and documentation are available.

Number of amino acids: 262

Molecular weight: 29499.56

Theoretical pI: 7.00

Amino acid composition: 
Ala (A)  17	  6.5%
Arg (R)   9	  3.4%
Asn (N)   7	  2.7%
Asp (D)   8	  3.1%
Cys (C)   3	  1.1%
Gln (Q)   2	  0.8%
Glu (E)  11	  4.2%
Gly (G)  19	  7.3%
His (H)   6	  2.3%
Ile (I)  19	  7.3%
Leu (L)  42	 16.0%
Lys (K)  10	  3.8%
Met (M)   3	  1.1%
Phe (F)  17	  6.5%
Pro (P)  18	  6.9%
Ser (S)  18	  6.9%
Thr (T)  18	  6.9%
Trp (W)   7	  2.7%
Tyr (Y)  15	  5.7%
Val (V)  13	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	      1400
Hydrogen    H	      2119
Nitrogen    N	       327
Oxygen      O	       361
Sulfur      S	         6

Formula: C1400H2119N327O361S6
Total number of atoms: 4213

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60975
Abs 0.1% (=1 g/l)   2.067, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   2.063, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.97
This classifies the protein as stable.



Aliphatic index: 111.68

Grand average of hydropathicity (GRAVY): 0.402