ProtParam
User-provided sequence:
10 20 30 40 50 60
MNYGVEKLKL KYSKHGCITC KIRKKRCSED KPVCRDCRRL SFPCIYISES VDKQSLKKIK
70 80 90 100 110 120
ADIQHQLISK KRKHAPDSAQ KAAVATRTRR VGSDEQDNQV YLSKPLEDCI SQKLDSMGLQ
130 140 150 160 170 180
LYNYYRSHLA NIISIAPMNQ NYYLNIFLPM AHENDGILFA ILAWSANHLS ISSSNELRKD
190 200 210 220 230 240
EIFVNLANKY TYMSLSHLKT NEGSSACAKL GFLYSLAQIL ILCGSEICQG DVKFWKILLN
250 260 270 280 290 300
IGKNLIENHV GKDVSRILTT TTEEPSLEER IIFPNFNSVV KYWLIVNFIY HDILNFNTTS
310 320 330 340 350 360
FPIEQYEKFF QRDQNSLPSS ANFIESIDSP IEEIDPLIGI NKPILLLLGQ VTNLTRFLQT
370 380 390 400 410 420
MEQEEMLEHG DKILSLQVEI YKLQPSLMAL EHLDDEKKFY YLELFEIMKI STLMFFQLTL
430 440 450 460 470 480
LKIDKDSLEL QILRNKLDSK LDKVIGTFLE GSLCFPLFIY GVCIQVEDME KKIDLEAKFD
490 500 510 520
DILKRYKCYN FQNARLLIRK IWQNEADGIS EHDLVHMIDE LDYNINFA
References and
documentation are available.
Number of amino acids: 528
Molecular weight: 61223.86
Theoretical pI: 6.27
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 2762
Hydrogen H 4367
Nitrogen N 715
Oxygen O 802
Sulfur S 25
Formula: C
2762H
4367N
715O
802S
25
Total number of atoms: 8671
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 55530
Abs 0.1% (=1 g/l) 0.907, assuming all pairs of Cys residues form cystines
Ext. coefficient 54780
Abs 0.1% (=1 g/l) 0.895, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.30
This classifies the protein as unstable.
Aliphatic index: 102.50
Grand average of hydropathicity (GRAVY): -0.211