ProtParam
User-provided sequence:
10 20 30 40 50 60
MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK
70 80 90 100 110 120
NHSGKLVCKL CNTMHMSWSS VERHLGGKKH GLNVLRRGIS IEKSSLGREG QTTHDFRQQQ
130 140 150 160 170 180
KIIEAKQSLK NNGTIPVCKI ATVKNPKNGS VGLAIQVNYS SEVKENSVDS DDKAKVPPLI
190 200 210 220 230 240
RIVSGLELSD TKQKGKKFLV IAYEPFENIA IELPPNEILF SENNDMDNNN DGVDELNKKC
250 260
TFWDAISKLY YVQFFFKQAE QEQADV
References and
documentation are available.
Number of amino acids: 266
Molecular weight: 29920.79
Theoretical pI: 8.19
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1315
Hydrogen H 2093
Nitrogen N 369
Oxygen O 412
Sulfur S 8
Formula: C
1315H
2093N
369O
412S
8
Total number of atoms: 4197
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23170
Abs 0.1% (=1 g/l) 0.774, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.766, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.28
This classifies the protein as unstable.
Aliphatic index: 77.26
Grand average of hydropathicity (GRAVY): -0.682