ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNYNEALEFL SNLTKFGINL GLGRISELLK RLGNPQEKVK VVHIGGTNGK GSVCSLTASV 

        70         80         90        100        110        120 
LAEAGYKTGL FTSPHLSRYT ERYKINGKEI SPERVSQLVT EIRPLLLAMV EEGFEHPTEF 

       130        140        150        160        170        180 
EVSTAMAFKY FAEEEVDFVV LEVGLGGLID STNVVSRPEV VAITNVGMDH MDYLGNTITE 

       190        200        210        220        230        240 
IARVKAGIIK PGVPVVTAAT GEALEVIKEV AKEKNSPLLD VNKDIVLNLK EKSLKGQVFD 

       250        260        270        280        290        300 
LIYRGETYPE IKIRLLGAHQ LLNAKTSFGI LNILREKYNI PVEAIYRGFS NATWSGRFEY 

       310        320        330        340        350        360 
IPGNPPVLLD GAHNHDGVRA LRQAFTDYFP GVKPVLLLGV LADKEREKVV AELLDLPLEV 

       370        380        390        400        410        420 
VVTRPPTPRA GDITVLARLF KEKGVRVKVI EEPTEALSYG LEIAQEYQTL LLITGSLYMI 

       430 
GEIRPLLLQD RD 

References and documentation are available.

Number of amino acids: 432

Molecular weight: 47738.26

Theoretical pI: 5.71

Amino acid composition: 
Ala (A)  28	  6.5%
Arg (R)  22	  5.1%
Asn (N)  19	  4.4%
Asp (D)  15	  3.5%
Cys (C)   1	  0.2%
Gln (Q)   8	  1.9%
Glu (E)  41	  9.5%
Gly (G)  36	  8.3%
His (H)   7	  1.6%
Ile (I)  28	  6.5%
Leu (L)  55	 12.7%
Lys (K)  27	  6.2%
Met (M)   6	  1.4%
Phe (F)  15	  3.5%
Pro (P)  22	  5.1%
Ser (S)  19	  4.4%
Thr (T)  25	  5.8%
Trp (W)   1	  0.2%
Tyr (Y)  15	  3.5%
Val (V)  42	  9.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2162
Hydrogen    H	      3481
Nitrogen    N	       567
Oxygen      O	       631
Sulfur      S	         7

Formula: C2162H3481N567O631S7
Total number of atoms: 6848

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27850
Abs 0.1% (=1 g/l)   0.583, assuming all pairs of Cys residues form cystines


Ext. coefficient    27850
Abs 0.1% (=1 g/l)   0.583, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.31
This classifies the protein as stable.



Aliphatic index: 109.61

Grand average of hydropathicity (GRAVY): -0.005