ProtParam
User-provided sequence:
10 20 30 40 50 60
MNYNLSKYPD DVSRLFKPRP PLSYKRPTDY PYAKRQTNPN ITGVANLLST SLKHYMEEFP
70 80 90 100 110 120
EGSPNNHLQR YEDIKLSKIK NAQLLDRRLQ NWNPNVDPHI KDTDPYRTIF IGRLPYDLDE
130 140 150 160 170 180
IELQKYFVKF GEIEKIRIVK DKITQKSKGY AFIVFKDPIS SKMAFKEIGV HRGIQIKDRI
190 200 210 220 230 240
CIVDIERGRT VKYFKPRRLG GGLGGRGYSN RDSRLPGRFA SASTSNPAER NYAPRLPRRE
250 260 270 280 290 300
TSSSAYSADR YGSSTLDARY RGNRPLLSAA TPTAAVTSVY KSRNSRTRES QPAPKEAPDY
References and
documentation are available.
Number of amino acids: 300
Molecular weight: 34447.07
Theoretical pI: 9.97
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 1531
Hydrogen H 2424
Nitrogen N 450
Oxygen O 449
Sulfur S 4
Formula: C
1531H
2424N
450O
449S
4
Total number of atoms: 4858
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33810
Abs 0.1% (=1 g/l) 0.982, assuming all pairs of Cys residues form cystines
Ext. coefficient 33810
Abs 0.1% (=1 g/l) 0.982, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.78
This classifies the protein as unstable.
Aliphatic index: 68.63
Grand average of hydropathicity (GRAVY): -0.880