ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPADHEQVPT TSSHMMKTTK RGRPFLKDTL DLFATLVVSL ELAPNKQFFR TFPNSFTTDQ 

        70         80         90        100        110        120 
AAANLASLKF SQSNRGPDPR DPTRIVTTTT TTTFSMTRDM AKAMCQHFMD ARLIENAADP 

       130        140        150        160        170        180 
SLNLFKDRGV YQLTPKGLHV LERFISRNGM NSDHLQQVFA TQPICIKLLH LERRSADDEI 

       190        200        210        220        230        240 
IVTQSVITAL FRRFVGRSPN YQSAANQPVD PFQRYNERSK GIGLVDVTER AQPLLGKAQQ 

       250        260        270        280        290        300 
QHKYCFQAVH ALEWLCDFTS VVGREEAAEM AAQFVRFGLI TLVSDKRKNN DSAIIFTVRG 

       310        320        330        340        350        360 
SAPGGNSPVS QQGEFRCTSK AIYKITEEGK RVAHWDQRVD SPSASNTNVS TERSSVDDHH 

       370        380        390        400        410        420 
DHDKKSKESN TERLRYILED PPLRSLFREF LRGNFCEENL SFWLEVQDFK RKFNITSSAQ 

       430        440        450        460        470        480 
ANNPRGARNT PGQAAMERHH ESLINTVFVI YNTYLAPSSQ CELNIDHNLR NELAKYLEDV 

       490        500        510        520        530        540 
VTNLTGKAFS GRVEPEQANA FNATQLQNMI RLYERIQTHV FRLMATDSVP KFIKTPKFLA 

       550        560        570        580        590        600 
MRDWDDDFDP TDNDIKQLSA NNPTMPPGLR QEETGGAYIT ISQQGSEREH QNRTGVAQQQ 


ETPATP 

References and documentation are available.

Number of amino acids: 606

Molecular weight: 68672.13

Theoretical pI: 7.69

Amino acid composition: 
Ala (A)  47	  7.8%
Arg (R)  45	  7.4%
Asn (N)  36	  5.9%
Asp (D)  36	  5.9%
Cys (C)   7	  1.2%
Gln (Q)  38	  6.3%
Glu (E)  36	  5.9%
Gly (G)  26	  4.3%
His (H)  17	  2.8%
Ile (I)  25	  4.1%
Leu (L)  46	  7.6%
Lys (K)  28	  4.6%
Met (M)  14	  2.3%
Phe (F)  34	  5.6%
Pro (P)  32	  5.3%
Ser (S)  43	  7.1%
Thr (T)  48	  7.9%
Trp (W)   4	  0.7%
Tyr (Y)  11	  1.8%
Val (V)  33	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      3003
Hydrogen    H	      4721
Nitrogen    N	       881
Oxygen      O	       927
Sulfur      S	        21

Formula: C3003H4721N881O927S21
Total number of atoms: 9553

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38765
Abs 0.1% (=1 g/l)   0.564, assuming all pairs of Cys residues form cystines


Ext. coefficient    38390
Abs 0.1% (=1 g/l)   0.559, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.26
This classifies the protein as unstable.



Aliphatic index: 69.24

Grand average of hydropathicity (GRAVY): -0.618