ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPAFGDLTMD QEMEPAIMTS TSDKREHLYK ILVIGELGTG KTSFIKRYVH QFFSQNYRAT 

        70         80         90        100        110        120 
IGVDFALKVL QWDANTIVRL QLWDIAGQER FGNMTRVYYK EAVGAFIVFD VTRSGTFDCV 

       130        140        150        160        170        180 
SKWKEDLDSK VQLPDGSPIP CILLANKCDQ EKQGIITQPE KMDEYVRENG FAGWFETSAK 

       190        200        210        220        230 
ENINIDEAAR ALVNKILIND KLISADLADG DKFNLSAADA TGSDAKNKCS C 

References and documentation are available.

Number of amino acids: 231

Molecular weight: 25886.42

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  20	  8.7%
Arg (R)   9	  3.9%
Asn (N)  11	  4.8%
Asp (D)  20	  8.7%
Cys (C)   5	  2.2%
Gln (Q)  10	  4.3%
Glu (E)  14	  6.1%
Gly (G)  15	  6.5%
His (H)   2	  0.9%
Ile (I)  17	  7.4%
Leu (L)  17	  7.4%
Lys (K)  18	  7.8%
Met (M)   6	  2.6%
Phe (F)  12	  5.2%
Pro (P)   6	  2.6%
Ser (S)  13	  5.6%
Thr (T)  13	  5.6%
Trp (W)   4	  1.7%
Tyr (Y)   6	  2.6%
Val (V)  13	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1149
Hydrogen    H	      1799
Nitrogen    N	       305
Oxygen      O	       353
Sulfur      S	        11

Formula: C1149H1799N305O353S11
Total number of atoms: 3617

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31190
Abs 0.1% (=1 g/l)   1.205, assuming all pairs of Cys residues form cystines


Ext. coefficient    30940
Abs 0.1% (=1 g/l)   1.195, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.76
This classifies the protein as stable.



Aliphatic index: 82.38

Grand average of hydropathicity (GRAVY): -0.290