ProtParam
User-provided sequence:
10 20 30 40 50 60
MPAPHGGILQ DLIARDALKK NELLSEAQSS DILVWNLTPR QLCDIELILN GGFSPLTGFL
70 80 90 100 110 120
NENDYSSVVT DSRLADGTLW TIPITLDVDE AFANQIKPDT RIALFQDDEI PIAILTVQDV
130 140 150 160 170 180
YKPNKTIEAE KVFRGDPEHP AISYLFNVAG DYYVGGSLEA IQLPQHYDYP GLRKTPAQLR
190 200 210 220 230 240
LEFQSRQWDR VVAFQTRNPM HRAHRELTVR AAREANAKVL IHPVVGLTKP GDIDHHTRVR
250 260 270 280 290 300
VYQEIIKRYP NGIAFLSLLP LAMRMSGDRE AVWHAIIRKN YGASHFIVGR DHAGPGKNSK
310 320 330 340 350 360
GVDFYGPYDA QELVESYKHE LDIEVVPFRM VTYLPDEDRY APIDQIDTTK TRTLNISGTE
370 380 390 400 410 420
LRRRLRVGGE IPEWFSYPEV VKILRESNPP RPKQGFSIVL GNSLTVSREQ LSIALLSTFL
430 440 450 460 470 480
QFGGGRYYKI FEHNNKTELL SLIQDFIGSG SGLIIPNQWE DDKDSVVGKQ NVYLLDTSSS
490 500 510
ADIQLESADE PISHIVQKVV LFLEDNGFFV F
References and
documentation are available.
Number of amino acids: 511
Molecular weight: 57724.51
Theoretical pI: 5.52
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2603
Hydrogen H 4061
Nitrogen N 707
Oxygen O 767
Sulfur S 6
Formula: C
2603H
4061N
707O
767S
6
Total number of atoms: 8144
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 61310
Abs 0.1% (=1 g/l) 1.062, assuming all pairs of Cys residues form cystines
Ext. coefficient 61310
Abs 0.1% (=1 g/l) 1.062, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.92
This classifies the protein as unstable.
Aliphatic index: 95.95
Grand average of hydropathicity (GRAVY): -0.288