ProtParam
User-provided sequence:
10 20 30 40 50 60
MPATLHDSTK ILSLNTGAQI PQIGLGTWQS KENDAYKAVL TALKDGYRHI DTAAIYRNED
70 80 90 100 110 120
QVGQAIKDSG VPREEIFVTT KLWCTQHHEP EVALDQSLKR LGLDYVDLYL MHWPARLDPA
130 140 150 160 170 180
YIKNEDILSV PTKKDGSRAV DITNWNFIKT WELMQELPKT GKTKAVGVSN FSINNLKDLL
190 200 210 220 230 240
ASQGNKLTPA ANQVEIHPLL PQDELINFCK SKGIVVEAYS PLGSTDAPLL KEPVILEIAK
250 260 270 280 290 300
KNNVQPGHVV ISWHVQRGYV VLPKSVNPDR IKTNRKIFTL STEDFEAINN ISKEKGEKRV
310
VHPNWSPFEV FK
References and
documentation are available.
Number of amino acids: 312
Molecular weight: 35079.16
Theoretical pI: 7.75
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1581
Hydrogen H 2505
Nitrogen N 427
Oxygen O 464
Sulfur S 5
Formula: C
1581H
2505N
427O
464S
5
Total number of atoms: 4982
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50545
Abs 0.1% (=1 g/l) 1.441, assuming all pairs of Cys residues form cystines
Ext. coefficient 50420
Abs 0.1% (=1 g/l) 1.437, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.03
This classifies the protein as stable.
Aliphatic index: 93.72
Grand average of hydropathicity (GRAVY): -0.394