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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPATLHDSTK ILSLNTGAQI PQIGLGTWQS KENDAYKAVL TALKDGYRHI DTAAIYRNED

70 80 90 100 110 120
QVGQAIKDSG VPREEIFVTT KLWCTQHHEP EVALDQSLKR LGLDYVDLYL MHWPARLDPA

130 140 150 160 170 180
YIKNEDILSV PTKKDGSRAV DITNWNFIKT WELMQELPKT GKTKAVGVSN FSINNLKDLL

190 200 210 220 230 240
ASQGNKLTPA ANQVEIHPLL PQDELINFCK SKGIVVEAYS PLGSTDAPLL KEPVILEIAK

250 260 270 280 290 300
KNNVQPGHVV ISWHVQRGYV VLPKSVNPDR IKTNRKIFTL STEDFEAINN ISKEKGEKRV

310
VHPNWSPFEV FK


References and documentation are available.

Number of amino acids: 312 Molecular weight: 35079.16 Theoretical pI: 7.75
Amino acid composition: 
Ala (A) 20 6.4% Arg (R) 10 3.2% Asn (N) 19 6.1% Asp (D) 18 5.8% Cys (C) 2 0.6% Gln (Q) 13 4.2% Glu (E) 19 6.1% Gly (G) 16 5.1% His (H) 9 2.9% Ile (I) 22 7.1% Leu (L) 30 9.6% Lys (K) 28 9.0% Met (M) 3 1.0% Phe (F) 8 2.6% Pro (P) 19 6.1% Ser (S) 18 5.8% Thr (T) 19 6.1% Trp (W) 7 2.2% Tyr (Y) 8 2.6% Val (V) 24 7.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 38 Atomic composition: Carbon C 1581 Hydrogen H 2505 Nitrogen N 427 Oxygen O 464 Sulfur S 5 Formula: C1581H2505N427O464S5 Total number of atoms: 4982 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 50545 Abs 0.1% (=1 g/l) 1.441, assuming all pairs of Cys residues form cystines Ext. coefficient 50420 Abs 0.1% (=1 g/l) 1.437, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 33.03 This classifies the protein as stable. Aliphatic index: 93.72 Grand average of hydropathicity (GRAVY): -0.394