ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPATLKNSSA TLKLNTGASI PVLGFGTWRS VDNNGYHSVI AALKAGYRHI DAAAIYLNEE 

        70         80         90        100        110        120 
EVGRAIKDSG VPREEIFITT KLWGTEQRDP EAALNKSLKR LGLDYVDLYL MHWPVPLKTD 

       130        140        150        160        170        180 
RVTDGNVLCI PTLEDGTVDI DTKEWNFIKT WELMQELPKT GKTKAVGVSN FSINNIKELL 

       190        200        210        220        230        240 
ESPNNKVVPA TNQIEIHPLL PQDELIAFCK EKGIVVEAYS PFGSANAPLL KEQAIIDMAK 

       250        260        270        280        290        300 
KHGVEPAQLI ISWSIQRGYV VLAKSVNPER IVSNFKIFTL PEDDFKTISN LSKVHGTKRV 

       310 
VDMKWGSFPI FQ 

References and documentation are available.

Number of amino acids: 312

Molecular weight: 34755.02

Theoretical pI: 6.62

Amino acid composition: 
Ala (A)  22	  7.1%
Arg (R)  10	  3.2%
Asn (N)  18	  5.8%
Asp (D)  16	  5.1%
Cys (C)   2	  0.6%
Gln (Q)   8	  2.6%
Glu (E)  21	  6.7%
Gly (G)  19	  6.1%
His (H)   6	  1.9%
Ile (I)  25	  8.0%
Leu (L)  29	  9.3%
Lys (K)  26	  8.3%
Met (M)   5	  1.6%
Phe (F)  11	  3.5%
Pro (P)  18	  5.8%
Ser (S)  19	  6.1%
Thr (T)  19	  6.1%
Trp (W)   7	  2.2%
Tyr (Y)   7	  2.2%
Val (V)  24	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1574
Hydrogen    H	      2493
Nitrogen    N	       413
Oxygen      O	       458
Sulfur      S	         7

Formula: C1574H2493N413O458S7
Total number of atoms: 4945

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49055
Abs 0.1% (=1 g/l)   1.411, assuming all pairs of Cys residues form cystines


Ext. coefficient    48930
Abs 0.1% (=1 g/l)   1.408, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.03
This classifies the protein as stable.



Aliphatic index: 96.86

Grand average of hydropathicity (GRAVY): -0.186