ProtParam
User-provided sequence:
10 20 30 40 50 60
MPCLLPSLLR ATRAALPLLS PPRVVAASAS QRLLSAPAQP AASRSSMDSA EELLAPLRLA
70 80 90 100 110 120
VRQQGDFVRK LKEDKAPQVD VDRAVAELKA RKRVLEAKEL ALQPKDDIVD RAKMEDTLKR
130 140 150 160 170 180
RFFYDQAFAI YGGVSGLYDF GPVGCALKNN IIQAWRQHFI QEEQILEIDC TMLTPEPVLK
190 200 210 220 230 240
TSGHVDKFAD FMVKDVKNGE CFRADHLLKA HLQKLMSDKK CSAEKKSEME SVLAQLDNYG
250 260 270 280 290 300
QQELADLFVN YNVKSPTTGN DLSPPVPFNL MFQTFIGPGG NMPGYLRPET AQGIFLNFKR
310 320 330 340 350 360
LLEFNQGKLP FAAAQIGNSF RNEISPRSGL IRVREFTMAE IEHFVDPTEK DHPKFQSVAD
370 380 390 400 410 420
LCLYLYSAKA QVTGQSARKM RLGDAVEQGV INNSVLGYFI GRIYLYLTKV GISPDKLRFR
430 440 450 460 470 480
QHMENEMAHY ACDCWDAESK TSYGWIEIVG CADRSCYDLS CHARATKVPL VAEKPLKEPK
490 500 510 520 530 540
TVNVVQFEPN KGAVGKAYKK DAKLVLEYLS ACDECYISEM ELLLSEKGEF TIETEGKTFQ
550 560 570 580 590 600
LTKDMVSVKR FQKTLHVEEV VPSVIEPSFG LGRIMYTILE HTFHVREGDE QRTFFSFPAV
610 620 630 640 650 660
VAPFKCSVLP LSQNQEFMPF VKELSEALTR NGVSHKVDDS SGSIGRRYAR TDEIGVAFGI
670 680 690 700 710 720
TIDFDTVNKT PHTATLRDRD SMRQIRAEVS ELPSVVRDLA NGNITWADVE ARYPLFEGQE
TGKKETVEE
References and
documentation are available.
Number of amino acids: 729
Molecular weight: 81850.74
Theoretical pI: 6.24
Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 93
Atomic composition:
Carbon C 3643
Hydrogen H 5759
Nitrogen N 995
Oxygen O 1083
Sulfur S 32
Formula: C
3643H
5759N
995O
1083S
32
Total number of atoms: 11512
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52675
Abs 0.1% (=1 g/l) 0.644, assuming all pairs of Cys residues form cystines
Ext. coefficient 51800
Abs 0.1% (=1 g/l) 0.633, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.26
This classifies the protein as unstable.
Aliphatic index: 83.21
Grand average of hydropathicity (GRAVY): -0.310