ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPDNNTEQLQ GSPSSDQRLR VDWDNGNHFD VSPDRYAPHL SEFYPIVNSK RPVASSAGSE 

        70         80         90        100        110        120 
NNDHLDDMNH LRSSKVYSKA RRASSITSGT STINDLQTLI TKRDVKETQE ALSTLLRNSN 

       130        140        150        160        170        180 
AYSDSLLKTS QNGAEIAHSL ENIAKLKGCN DETAEKLLSA SGLFYLLSNH QLIMSKYFND 

       190        200        210        220        230        240 
LLGDNLIDDI DEFELQTKIM ENKFKAQSKE QSLKLKLQER HNFDISKRKI RNLISYRESL 

       250        260        270        280        290        300 
SSLQARLDQL ETLKHDFYMD SYELVENTCN KVLSKVATVS RAQVEISENI ARKGWSGGGL 

       310        320        330        340        350        360 
DELLCDADDP FSKKADGPYG TIGGDGETAG EAYNSDEETG GNDVVLNELL EGTSQPSTSK 

       370        380        390        400        410        420 
TSLPKSKGSS TVSTPNHSQS SSNKDGVRNN GGGKNGEDED TDNLMGTENS FSLPPTRNSA 

       430        440        450 
EETTQTFKQL SIKEDNDNHS SDTDGMQDQS SNI 

References and documentation are available.

Number of amino acids: 453

Molecular weight: 49975.52

Theoretical pI: 4.89

Amino acid composition: 
Ala (A)  22	  4.9%
Arg (R)  18	  4.0%
Asn (N)  37	  8.2%
Asp (D)  39	  8.6%
Cys (C)   3	  0.7%
Gln (Q)  20	  4.4%
Glu (E)  33	  7.3%
Gly (G)  29	  6.4%
His (H)  10	  2.2%
Ile (I)  18	  4.0%
Leu (L)  45	  9.9%
Lys (K)  31	  6.8%
Met (M)   7	  1.5%
Phe (F)  11	  2.4%
Pro (P)  13	  2.9%
Ser (S)  60	 13.2%
Thr (T)  29	  6.4%
Trp (W)   2	  0.4%
Tyr (Y)  11	  2.4%
Val (V)  15	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2125
Hydrogen    H	      3383
Nitrogen    N	       617
Oxygen      O	       755
Sulfur      S	        10

Formula: C2125H3383N617O755S10
Total number of atoms: 6890

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27515
Abs 0.1% (=1 g/l)   0.551, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.548, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.73
This classifies the protein as unstable.



Aliphatic index: 68.70

Grand average of hydropathicity (GRAVY): -0.874