ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPDYDNYTTP LSSRYASKEM SATFSLRNRF STWRKLWLNL AIAEKELGLT VVTDEAIEQM 

        70         80         90        100        110        120 
RKHVEITDDE IAKASAQEAI VRHDVMAHVH TFGETCPAAA GIIHLGATSC FVTDNADLIF 

       130        140        150        160        170        180 
IRDAYDIIIP KLVNVINRLA KFAMEYKDLP VLGWTHFQPA QLTTLGKRAT LWIQELLWDL 

       190        200        210        220        230        240 
RNFERARNDI GLRGVKGTTG TQASFLALFH GNHDKVEALD ERVTELLGFD KVYPVTGQTY 

       250        260        270        280        290        300 
SRKIDIDVLA PLSSFAATAH KMATDIRLLA NLKEVEEPFE KSQIGSSAMA YKRNPMRCER 

       310        320        330        340        350        360 
VCSLARHLGS LFSDAVQTAS VQWFERTLDD SAIRRISLPS AFLTADILLS TLLNISSGLV 

       370        380        390        400        410        420 
VYPKVIERRI KGELPFMATE NIIMAMVEKN ASRQEVHERI RVLSHQAAAV VKEEGGENDL 

       430        440        450        460        470        480 
IERVKRDEFF KPIWEELDSL LEPSTFVGRA PQQVEKFVQK DVNNALQPFQ KYLNDEQVKL 


NV 

References and documentation are available.

Number of amino acids: 482

Molecular weight: 54510.47

Theoretical pI: 6.01

Amino acid composition: 
Ala (A)  46	  9.5%
Arg (R)  31	  6.4%
Asn (N)  19	  3.9%
Asp (D)  28	  5.8%
Cys (C)   4	  0.8%
Gln (Q)  18	  3.7%
Glu (E)  37	  7.7%
Gly (G)  20	  4.1%
His (H)  12	  2.5%
Ile (I)  30	  6.2%
Leu (L)  50	 10.4%
Lys (K)  27	  5.6%
Met (M)  11	  2.3%
Phe (F)  22	  4.6%
Pro (P)  17	  3.5%
Ser (S)  29	  6.0%
Thr (T)  29	  6.0%
Trp (W)   7	  1.5%
Tyr (Y)  10	  2.1%
Val (V)  35	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2436
Hydrogen    H	      3868
Nitrogen    N	       670
Oxygen      O	       718
Sulfur      S	        15

Formula: C2436H3868N670O718S15
Total number of atoms: 7707

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53650
Abs 0.1% (=1 g/l)   0.984, assuming all pairs of Cys residues form cystines


Ext. coefficient    53400
Abs 0.1% (=1 g/l)   0.980, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.71
This classifies the protein as unstable.



Aliphatic index: 95.33

Grand average of hydropathicity (GRAVY): -0.189