ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPEDTSYSNS FEDYYNNSHA ISPYKDSFYK EMTPSKPNVR FGDDDVNIFD QRKKVNEINK 

        70         80         90        100        110        120 
NNTVKRSIPS SISTTITPNK SSLKSPRGKR ASKNSFDNET KLESKNETLK EVNDAVNRCY 

       130        140        150        160        170        180 
ALCNIPTKHV SINSISDLAQ TFETLAVGIT HETNRKAECE RSKNAIDSLY YHEQLEKKEL 

       190        200        210        220        230        240 
NEKSLQMAID HLLKVTKQNL RQADDGNKLK ETEALKSFIE EIEEVDDNKI SINSLQQQLL 

       250        260        270        280        290        300 
EEKTANNILR RDYYKLQERG RRLCHEFQEL QDDYSKQMKQ KEYEVQKLKN EIKVLLNMND 

       310        320        330        340        350        360 
NLKAEKAHYS QKEKQYFQKY TYIEKYMNHV KEEYNRKEDE CKKLNFIIDK SMKKIEHLER 

       370        380        390        400        410        420 
SLQTQFTAQN SFSTAMIQEE GPKDAHLKDR YHKVKEFMEQ KLQTSKINDP SCSEAEALDN 

       430        440        450        460 
VLCLIESSMK TLDKNSKCYP IATKKCIKYV TDSPRLKENE HVTN 

References and documentation are available.

Number of amino acids: 464

Molecular weight: 54358.17

Theoretical pI: 7.89

Amino acid composition: 
Ala (A)  20	  4.3%
Arg (R)  18	  3.9%
Asn (N)  39	  8.4%
Asp (D)  28	  6.0%
Cys (C)   9	  1.9%
Gln (Q)  24	  5.2%
Glu (E)  48	 10.3%
Gly (G)   6	  1.3%
His (H)  12	  2.6%
Ile (I)  27	  5.8%
Leu (L)  36	  7.8%
Lys (K)  60	 12.9%
Met (M)  10	  2.2%
Phe (F)  13	  2.8%
Pro (P)  12	  2.6%
Ser (S)  39	  8.4%
Thr (T)  25	  5.4%
Trp (W)   0	  0.0%
Tyr (Y)  21	  4.5%
Val (V)  17	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      2363
Hydrogen    H	      3783
Nitrogen    N	       665
Oxygen      O	       765
Sulfur      S	        19

Formula: C2363H3783N665O765S19
Total number of atoms: 7595

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31790
Abs 0.1% (=1 g/l)   0.585, assuming all pairs of Cys residues form cystines


Ext. coefficient    31290
Abs 0.1% (=1 g/l)   0.576, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.42
This classifies the protein as unstable.



Aliphatic index: 67.89

Grand average of hydropathicity (GRAVY): -1.064