ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPEVETKIIP NEKEDEDEDG YIEEEDEDFQ PEKDKLGGGS DDSDASDGGD DYDDGVNRDK 

        70         80         90        100        110        120 
GRNKVDYSRI ESESGGLIKT RRARQAEEEY AKTHKYESLT VESIPAKVNS IWEELQEASK 

       130        140        150        160        170        180 
NRLLSSSGKV GSVLDGSKEA RSTTAAQQED KILIERNYKF AGETVHEKKW VSRSSAEGQE 

       190        200        210        220        230        240 
YLNSLKFKQQ APAAPVQLEK AVRTKSNESR QHLRRPLKRP PLLEQIISGG LRPKLTTLEK 

       250        260        270        280        290        300 
SQLDWASYVD RAGLNDELVL HNKDGFLARQ EFLQRVGSAE DERYKELRRQ QLAQQLQQDS 


EAS 

References and documentation are available.

Number of amino acids: 303

Molecular weight: 34343.71

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)  21	  6.9%
Arg (R)  22	  7.3%
Asn (N)  10	  3.3%
Asp (D)  24	  7.9%
Cys (C)   0	  0.0%
Gln (Q)  20	  6.6%
Glu (E)  38	 12.5%
Gly (G)  20	  6.6%
His (H)   4	  1.3%
Ile (I)  11	  3.6%
Leu (L)  27	  8.9%
Lys (K)  26	  8.6%
Met (M)   1	  0.3%
Phe (F)   5	  1.7%
Pro (P)  10	  3.3%
Ser (S)  28	  9.2%
Thr (T)  10	  3.3%
Trp (W)   3	  1.0%
Tyr (Y)   9	  3.0%
Val (V)  14	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      1477
Hydrogen    H	      2366
Nitrogen    N	       436
Oxygen      O	       505
Sulfur      S	         1

Formula: C1477H2366N436O505S1
Total number of atoms: 4785

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.871

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 67.80
This classifies the protein as unstable.



Aliphatic index: 69.24

Grand average of hydropathicity (GRAVY): -1.117