ProtParam
User-provided sequence:
10 20 30 40 50 60
MPFAQIVIGP PGSGKSTYCN GCSQFFNAIG RHSQVVNMDP ANDALPYPCA VDIRDFITLE
70 80 90 100 110 120
EIMQEQQLGP NGGLMYAVES LDNSIDLFIL QIKSLVEEEK AYLVFDCPGQ VELFTHHSSL
130 140 150 160 170 180
FNIFKKMEKE LDIRFCVVNL IDCFYMTSPS QYISILLLAL RSMLMMDLPH INVFSKIDML
190 200 210 220 230 240
KSYGELPFRL DYYTEVQDLD YLEPYIEKEG SSVLGKKYSK LTETIKELVS DFNLVSFEVL
250 260 270 280 290 300
SVDDKESMIN LQGVIDKANG YIFGASEVGG DTVWAEASRE GALIANYDIQ DRWIDNKEKY
310 320 330 340
DKEEEEKRTA LLKEQELQNK AVDVNEEDEW ENALKEWEEK QGMDFVR
References and
documentation are available.
Number of amino acids: 347
Molecular weight: 39724.10
Theoretical pI: 4.43
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1779
Hydrogen H 2749
Nitrogen N 441
Oxygen O 552
Sulfur S 18
Formula: C
1779H
2749N
441O
552S
18
Total number of atoms: 5539
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44725
Abs 0.1% (=1 g/l) 1.126, assuming all pairs of Cys residues form cystines
Ext. coefficient 44350
Abs 0.1% (=1 g/l) 1.116, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.88
This classifies the protein as unstable.
Aliphatic index: 90.43
Grand average of hydropathicity (GRAVY): -0.270