ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPFAQIVIGP PGSGKSTYCN GCSQFFNAIG RHSQVVNMDP ANDALPYPCA VDIRDFITLE 

        70         80         90        100        110        120 
EIMQEQQLGP NGGLMYAVES LDNSIDLFIL QIKSLVEEEK AYLVFDCPGQ VELFTHHSSL 

       130        140        150        160        170        180 
FNIFKKMEKE LDIRFCVVNL IDCFYMTSPS QYISILLLAL RSMLMMDLPH INVFSKIDML 

       190        200        210        220        230        240 
KSYGELPFRL DYYTEVQDLD YLEPYIEKEG SSVLGKKYSK LTETIKELVS DFNLVSFEVL 

       250        260        270        280        290        300 
SVDDKESMIN LQGVIDKANG YIFGASEVGG DTVWAEASRE GALIANYDIQ DRWIDNKEKY 

       310        320        330        340 
DKEEEEKRTA LLKEQELQNK AVDVNEEDEW ENALKEWEEK QGMDFVR 

References and documentation are available.

Number of amino acids: 347

Molecular weight: 39724.10

Theoretical pI: 4.43

Amino acid composition: 
Ala (A)  17	  4.9%
Arg (R)   9	  2.6%
Asn (N)  17	  4.9%
Asp (D)  26	  7.5%
Cys (C)   6	  1.7%
Gln (Q)  15	  4.3%
Glu (E)  36	 10.4%
Gly (G)  19	  5.5%
His (H)   4	  1.2%
Ile (I)  24	  6.9%
Leu (L)  35	 10.1%
Lys (K)  23	  6.6%
Met (M)  12	  3.5%
Phe (F)  17	  4.9%
Pro (P)  12	  3.5%
Ser (S)  24	  6.9%
Thr (T)   9	  2.6%
Trp (W)   4	  1.2%
Tyr (Y)  15	  4.3%
Val (V)  23	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1779
Hydrogen    H	      2749
Nitrogen    N	       441
Oxygen      O	       552
Sulfur      S	        18

Formula: C1779H2749N441O552S18
Total number of atoms: 5539

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44725
Abs 0.1% (=1 g/l)   1.126, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   1.116, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.88
This classifies the protein as unstable.



Aliphatic index: 90.43

Grand average of hydropathicity (GRAVY): -0.270