ProtParam
User-provided sequence:
10 20 30 40 50 60
MPFQDYFQKK KAAFINRNNK SNADASALRD INDININFAA KSKNYVFPLT KLPDELMQEV
70 80 90 100 110 120
FSHLPQPDRL QLCLVNKRLN KIATKLLYRR IYLNDSNVVK SDFMHLAINW TLLNLPSSLK
130 140 150 160 170 180
EEESRDIANC KLKKLIETLQ NNIHITEVIQ WIRINWDLDS TLQRSILSIL CNQGKSLQRL
190 200 210 220 230 240
ENVTDPACND IISNGHFSRS NVSSFDMAPP NSLPEMVVPE NYIPNLTKYL SQRISSRLSH
250 260 270 280 290 300
MTLFIDPLKL FNYLYPLDIK LQIIDLKLHW RREFYNNDYF VKKIRPGNPL TKLSEVFDKR
310 320 330 340 350 360
TLKILTIISW NDTLLKRETE MLKDFKEFEN LEDLSLISIK QDVHILVDLF SSLTNLKRLK
370 380 390 400 410 420
MDFLEEYVPE PTNPHIFLSI LLACSKLQFI DLRYDGLIPQ IINIQENKFQ LNQQCNCTNC
430 440 450 460 470 480
QIVFSDILKG KIFMFPEDYY IHDLQDIAAK DIFKMMKYLS LLPYSKACDA YPSVRTQPMN
490 500 510 520 530 540
LTNFVTKMNR NLLEYRNSKS QLVPKIVNNP HQHSTVTSTS TAHMSEPEMI IIDDDDDDDE
550 560 570 580 590 600
INAAIPPSSD DTAATISTDL ELPHESLTKR DIIMCYHALI HHFKSIYVTF LKSFPHLRFL
610 620 630 640 650 660
MLNDIPTIVM EENNERIFEP VFYHYDYKSN LYGWSKESNK NLENDSNNNN NNSDTIARIA
TVM
References and
documentation are available.
Number of amino acids: 663
Molecular weight: 77366.91
Theoretical pI: 6.25
Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 3476
Hydrogen H 5466
Nitrogen N 918
Oxygen O 1020
Sulfur S 29
Formula: C
3476H
5466N
918O
1020S
29
Total number of atoms: 10909
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 69385
Abs 0.1% (=1 g/l) 0.897, assuming all pairs of Cys residues form cystines
Ext. coefficient 68760
Abs 0.1% (=1 g/l) 0.889, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.44
This classifies the protein as unstable.
Aliphatic index: 97.06
Grand average of hydropathicity (GRAVY): -0.344