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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH PDKHPDNPSI

70 80 90 100 110 120
IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN KLIQKLEESE SSTIPTTTPH

130 140 150 160 170 180
PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN TQDQENASQH PYYDCSTLRI VLDNFLQSNN

190 200 210 220 230 240
KSNCLSHLRN QVFITLSANE IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG

250 260 270 280
NLIPTVKDIS PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD


References and documentation are available.

Number of amino acids: 283 Molecular weight: 33237.43 Theoretical pI: 5.71
Amino acid composition: 
Ala (A) 7 2.5% Arg (R) 14 4.9% Asn (N) 23 8.1% Asp (D) 24 8.5% Cys (C) 2 0.7% Gln (Q) 11 3.9% Glu (E) 15 5.3% Gly (G) 4 1.4% His (H) 15 5.3% Ile (I) 24 8.5% Leu (L) 37 13.1% Lys (K) 14 4.9% Met (M) 1 0.4% Phe (F) 9 3.2% Pro (P) 18 6.4% Ser (S) 19 6.7% Thr (T) 19 6.7% Trp (W) 3 1.1% Tyr (Y) 14 4.9% Val (V) 10 3.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 28 Atomic composition: Carbon C 1495 Hydrogen H 2312 Nitrogen N 406 Oxygen O 448 Sulfur S 3 Formula: C1495H2312N406O448S3 Total number of atoms: 4664 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 37485 Abs 0.1% (=1 g/l) 1.128, assuming all pairs of Cys residues form cystines Ext. coefficient 37360 Abs 0.1% (=1 g/l) 1.124, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.93 This classifies the protein as unstable. Aliphatic index: 96.78 Grand average of hydropathicity (GRAVY): -0.585