ProtParam
User-provided sequence:
10 20 30 40 50 60
MPGHELEDVI NQRLNLYDVL ELPTPLDVHT IYDDLPQIKR KYRTLALKYH PDKHPDNPSI
70 80 90 100 110 120
IHKFHLLSTA TNILTNADVR PHYDRWLIEF LRKTNDIERN KLIQKLEESE SSTIPTTTPH
130 140 150 160 170 180
PDLLQIQRHG ELLRKLKHFN LPYGDWKHLN TQDQENASQH PYYDCSTLRI VLDNFLQSNN
190 200 210 220 230 240
KSNCLSHLRN QVFITLSANE IYDIYFSERN NYSKDDSIII YTVFDTPITA QHVFRNWSSG
250 260 270 280
NLIPTVKDIS PLIPLHYYSD FNLETELNDD IARLVSNEPI LLD
References and
documentation are available.
Number of amino acids: 283
Molecular weight: 33237.43
Theoretical pI: 5.71
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1495
Hydrogen H 2312
Nitrogen N 406
Oxygen O 448
Sulfur S 3
Formula: C
1495H
2312N
406O
448S
3
Total number of atoms: 4664
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37485
Abs 0.1% (=1 g/l) 1.128, assuming all pairs of Cys residues form cystines
Ext. coefficient 37360
Abs 0.1% (=1 g/l) 1.124, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.93
This classifies the protein as unstable.
Aliphatic index: 96.78
Grand average of hydropathicity (GRAVY): -0.585