ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPGLKLVEAL EYRCDRLERL IGAGYSANSD VSVQLDELYN QLHRLYFQGL KYSQDLLQLF 

        70         80         90        100        110        120 
NTFMAEDIEN VGAPDDICIF ASCFDDIYTL YSAFDELNSQ YMEFCQISKS SLDQISFKDA 

       130        140        150        160        170 
NIETKQLKKL PELVDNCNIM ILRSIAILNR FIDWNIEVNG FFQFQKKRLL NLQKVIYST 

References and documentation are available.

Number of amino acids: 179

Molecular weight: 20846.87

Theoretical pI: 4.66

Amino acid composition: 
Ala (A)   9	  5.0%
Arg (R)   7	  3.9%
Asn (N)  12	  6.7%
Asp (D)  14	  7.8%
Cys (C)   5	  2.8%
Gln (Q)  12	  6.7%
Glu (E)  11	  6.1%
Gly (G)   6	  3.4%
His (H)   1	  0.6%
Ile (I)  15	  8.4%
Leu (L)  24	 13.4%
Lys (K)  10	  5.6%
Met (M)   4	  2.2%
Phe (F)  12	  6.7%
Pro (P)   3	  1.7%
Ser (S)  13	  7.3%
Thr (T)   4	  2.2%
Trp (W)   1	  0.6%
Tyr (Y)   9	  5.0%
Val (V)   7	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 17

Atomic composition:

Carbon      C	       940
Hydrogen    H	      1457
Nitrogen    N	       237
Oxygen      O	       280
Sulfur      S	         9

Formula: C940H1457N237O280S9
Total number of atoms: 2923

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19160
Abs 0.1% (=1 g/l)   0.919, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.907, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.05
This classifies the protein as unstable.



Aliphatic index: 101.34

Grand average of hydropathicity (GRAVY): -0.113