ProtParam
User-provided sequence:
10 20 30 40 50 60
MPGPVIEEVN EEALMDAIKE QMKLQKENDV VVEDVKDGDE DDDDVDDDDD EIADGMFLES
70 80 90 100 110 120
CCGFVIESIC FESGLGAGEN EASKQSRSEK KSRKAMLKLG MKPVTDVSRV TIKRSKNVLF
130 140 150 160 170 180
VISKPDVFKS PNSETYVIFG EAKIDDMSSQ LQAQAAQRFK MPDVASMIPN TDGSEAATVA
190 200 210 220 230
QEEEDDEDVD ETGVEAKDID LVMTQAGVSR PKAVKALKES NGDIVSAIME LTT
References and
documentation are available.
Number of amino acids: 233
Molecular weight: 25425.37
Theoretical pI: 4.21
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1082
Hydrogen H 1755
Nitrogen N 287
Oxygen O 387
Sulfur S 14
Formula: C
1082H
1755N
287O
387S
14
Total number of atoms: 3525
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 1615
Abs 0.1% (=1 g/l) 0.064, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.059, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.46
This classifies the protein as unstable.
Aliphatic index: 76.95
Grand average of hydropathicity (GRAVY): -0.479