ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPGSALLCCL LLLAGVKTSK GHSIRGDNNC THFPVSQTHM LRELRAAFSQ VKTFFQKKDQ 

        70         80         90        100        110        120 
LDNILLTDSL LQDFKGYLGC QALSEMIKFY LVEVMPQAEN HGPEIKEHLN SLGEKLKTLW 

       130        140        150        160        170 
IQLRRCHRFL PCENKSKAVE QVKNDFNKLQ DKGVYKAMNE FDIFINCIEA YVTLKMKN 

References and documentation are available.

Number of amino acids: 178

Molecular weight: 20425.85

Theoretical pI: 8.80

Amino acid composition: 
Ala (A)   9	  5.1%
Arg (R)   6	  3.4%
Asn (N)  11	  6.2%
Asp (D)   8	  4.5%
Cys (C)   7	  3.9%
Gln (Q)  10	  5.6%
Glu (E)  11	  6.2%
Gly (G)   9	  5.1%
His (H)   6	  3.4%
Ile (I)   8	  4.5%
Leu (L)  24	 13.5%
Lys (K)  18	 10.1%
Met (M)   6	  3.4%
Phe (F)  10	  5.6%
Pro (P)   5	  2.8%
Ser (S)   9	  5.1%
Thr (T)   7	  3.9%
Trp (W)   1	  0.6%
Tyr (Y)   4	  2.2%
Val (V)   9	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	       911
Hydrogen    H	      1454
Nitrogen    N	       248
Oxygen      O	       258
Sulfur      S	        13

Formula: C911H1454N248O258S13
Total number of atoms: 2884

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11835
Abs 0.1% (=1 g/l)   0.579, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.561, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.94
This classifies the protein as unstable.



Aliphatic index: 89.83

Grand average of hydropathicity (GRAVY): -0.270