ProtParam
User-provided sequence:
10 20 30 40 50 60
MPHDSTYPPV KISERPYHEM LLDAIDKTIA DGKNKIAFVS GDNPKHFITF EQLRKDAFAV
70 80 90 100 110 120
AMYLHSIGFK KDVAAVVLPN VWHYTSFFIG CAINGGAVSG ASALFTDYEL QRQFVDSRAK
130 140 150 160 170 180
VVFTYEDFLP KVLLAVKQSP NIQKIIVIPK PVGSSLPAGV VSWNEVVSTP VTALPQVPID
190 200 210 220 230 240
VHNDLLVLPY SSGTTGPPKG VMLSHYNFTS MISMYLAIDK SHILDVLDPN WDCYNEKALL
250 260 270 280 290 300
FLPFYHVYGF GLLNHCLLKG MTGIVMSHFE PNNFLTAVQN YKVRCLCLVP PIMVFLAKHP
310 320 330 340 350 360
ICDKFDLSSV QMIMAGAAPA GKDLIEELKR KYTNLKYIQQ GYGMTECSMA SHLPDLRNDQ
370 380 390 400 410 420
PYGSVGKLAS NLVMKIVEPG TDREQPVNQR GEICVRGPTI MLGYLGRPEA TASTVIDGWL
430 440 450 460 470 480
HTGDIGYLNE DGNLFIVDRL KELIKVKGLQ VPPAELEDLL LSHPKIRDCA VIGIPDAKAG
490 500 510 520 530 540
ELPKAFVVRA DNTLTEQEVK DFVKPKVSPY KQLEGGVEFI EEIPKSAAGK ILRRFLRDRS
SAKL
References and
documentation are available.
Number of amino acids: 544
Molecular weight: 60306.11
Theoretical pI: 6.94
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 2741
Hydrogen H 4312
Nitrogen N 708
Oxygen O 772
Sulfur S 24
Formula: C
2741H
4312N
708O
772S
24
Total number of atoms: 8557
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52300
Abs 0.1% (=1 g/l) 0.867, assuming all pairs of Cys residues form cystines
Ext. coefficient 51800
Abs 0.1% (=1 g/l) 0.859, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.22
This classifies the protein as stable.
Aliphatic index: 97.63
Grand average of hydropathicity (GRAVY): -0.006