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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPHDSTYPPV KISERPYHEM LLDAIDKTIA DGKNKIAFVS GDNPKHFITF EQLRKDAFAV

70 80 90 100 110 120
AMYLHSIGFK KDVAAVVLPN VWHYTSFFIG CAINGGAVSG ASALFTDYEL QRQFVDSRAK

130 140 150 160 170 180
VVFTYEDFLP KVLLAVKQSP NIQKIIVIPK PVGSSLPAGV VSWNEVVSTP VTALPQVPID

190 200 210 220 230 240
VHNDLLVLPY SSGTTGPPKG VMLSHYNFTS MISMYLAIDK SHILDVLDPN WDCYNEKALL

250 260 270 280 290 300
FLPFYHVYGF GLLNHCLLKG MTGIVMSHFE PNNFLTAVQN YKVRCLCLVP PIMVFLAKHP

310 320 330 340 350 360
ICDKFDLSSV QMIMAGAAPA GKDLIEELKR KYTNLKYIQQ GYGMTECSMA SHLPDLRNDQ

370 380 390 400 410 420
PYGSVGKLAS NLVMKIVEPG TDREQPVNQR GEICVRGPTI MLGYLGRPEA TASTVIDGWL

430 440 450 460 470 480
HTGDIGYLNE DGNLFIVDRL KELIKVKGLQ VPPAELEDLL LSHPKIRDCA VIGIPDAKAG

490 500 510 520 530 540
ELPKAFVVRA DNTLTEQEVK DFVKPKVSPY KQLEGGVEFI EEIPKSAAGK ILRRFLRDRS


SAKL


References and documentation are available.

Number of amino acids: 544 Molecular weight: 60306.11 Theoretical pI: 6.94
Amino acid composition: 
Ala (A) 37 6.8% Arg (R) 18 3.3% Asn (N) 21 3.9% Asp (D) 32 5.9% Cys (C) 9 1.7% Gln (Q) 16 2.9% Glu (E) 26 4.8% Gly (G) 37 6.8% His (H) 15 2.8% Ile (I) 35 6.4% Leu (L) 56 10.3% Lys (K) 39 7.2% Met (M) 15 2.8% Phe (F) 24 4.4% Pro (P) 38 7.0% Ser (S) 32 5.9% Thr (T) 22 4.0% Trp (W) 4 0.7% Tyr (Y) 20 3.7% Val (V) 48 8.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 58 Total number of positively charged residues (Arg + Lys): 57 Atomic composition: Carbon C 2741 Hydrogen H 4312 Nitrogen N 708 Oxygen O 772 Sulfur S 24 Formula: C2741H4312N708O772S24 Total number of atoms: 8557 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 52300 Abs 0.1% (=1 g/l) 0.867, assuming all pairs of Cys residues form cystines Ext. coefficient 51800 Abs 0.1% (=1 g/l) 0.859, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.22 This classifies the protein as stable. Aliphatic index: 97.63 Grand average of hydropathicity (GRAVY): -0.006