ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPHRIMLIPV GSGVGLTSVS LGLLHACQQK GIKVGHFKPI SQPSRNSLAK KNKTIDAISL 

        70         80         90        100        110        120 
SQNSTSVSLS YVEGKMGDGL HDVVLEEIVA NFNAFKEDHD VIIIEGLVPT PRQPYAGRIN 

       130        140        150        160        170        180 
RDVAQALGAN IVLVASPGND SAEEFEDRLE VSAETYGGIK NRHLIGCVIN KLNCPDQEEF 

       190        200        210        220        230        240 
GLLPKEEEIE NDFNLNHWLD LSIFKGRHFD LLGTVQWEID LIAPRVCDIS NYLEAKVINN 

       250        260        270        280        290        300 
GDMEHRRFRS VAFCARTVSN LVSYLVPGRL IVTPGDRTDI IIAACLSALN GTKLGGLILT 

       310        320        330        340        350        360 
NGFEPSEQVW ELCKQALDAG LPVLSVPWDT WQTSRHIMGF NPEIPKDDIQ RQDKVKQHVA 

       370        380        390        400        410        420 
DNLSSSWLES LTSKNNSYNL LSPPAFRHKL TELARQANKL IVLPEGTDIR TIKAAAICCE 

       430        440        450        460        470        480 
RNIARCQLIG NKEEILQIAE HQGINLPDNL LITDPDSIRD NYVGPMVELR KNKGLTDVIA 

       490        500        510        520        530        540 
LENLKDNVVL ATMMLQLGQV DGLVSGAVNT TANTIRPALQ LIKTAPDSSL VSSVFFMLLP 

       550        560        570        580        590        600 
DQVLVYGDCA INPEPNAEQL ADIAIQSADS ARAFDIEPRV AMISYSTGRS GHGADVEKVA 

       610        620        630        640        650        660 
KATEIAKAKR PDLIIDGPLQ YDAAIMENVA KKKAPNSPVA GKATVFIFPD LNTGNTTYKA 

       670        680        690        700 
VQRSADLVSI GPMLQGMNKP VNDLSRGALV DDIVYTIALT AIQATM 

References and documentation are available.

Number of amino acids: 706

Molecular weight: 77000.22

Theoretical pI: 5.45

Amino acid composition: 
Ala (A)  61	  8.6%
Arg (R)  31	  4.4%
Asn (N)  44	  6.2%
Asp (D)  46	  6.5%
Cys (C)  11	  1.6%
Gln (Q)  28	  4.0%
Glu (E)  38	  5.4%
Gly (G)  46	  6.5%
His (H)  14	  2.0%
Ile (I)  57	  8.1%
Leu (L)  74	 10.5%
Lys (K)  37	  5.2%
Met (M)  14	  2.0%
Phe (F)  18	  2.5%
Pro (P)  37	  5.2%
Ser (S)  46	  6.5%
Thr (T)  33	  4.7%
Trp (W)   6	  0.8%
Tyr (Y)  12	  1.7%
Val (V)  53	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      3402
Hydrogen    H	      5492
Nitrogen    N	       942
Oxygen      O	      1038
Sulfur      S	        25

Formula: C3402H5492N942O1038S25
Total number of atoms: 10899

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51505
Abs 0.1% (=1 g/l)   0.669, assuming all pairs of Cys residues form cystines


Ext. coefficient    50880
Abs 0.1% (=1 g/l)   0.661, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.75
This classifies the protein as stable.



Aliphatic index: 102.78

Grand average of hydropathicity (GRAVY): -0.083