ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPIMLEDYQK NFLELAIECQ ALRFGSFKLK SGRESPYFFN LGLFNTGKLL SNLATAYAIA 

        70         80         90        100        110        120 
IIQSDLKFDV IFGPAYKGIP LAAIVCVKLA EIGGSKFQNI QYAFNRKEAK DHGEGGIIVG 

       130        140        150        160        170        180 
SALENKRILI IDDVMTAGTA INEAFEIISN AKGQVVGSII ALDRQEVVST DDKEGLSATQ 

       190        200        210        220 
TVSKKYGIPV LSIVSLIHII TYLEGRITAE EKSKIEQYLQ TYGASA 

References and documentation are available.

Number of amino acids: 226

Molecular weight: 24664.50

Theoretical pI: 5.80

Amino acid composition: 
Ala (A)  22	  9.7%
Arg (R)   6	  2.7%
Asn (N)   9	  4.0%
Asp (D)   9	  4.0%
Cys (C)   2	  0.9%
Gln (Q)  10	  4.4%
Glu (E)  16	  7.1%
Gly (G)  19	  8.4%
His (H)   2	  0.9%
Ile (I)  27	 11.9%
Leu (L)  21	  9.3%
Lys (K)  17	  7.5%
Met (M)   3	  1.3%
Phe (F)  11	  4.9%
Pro (P)   5	  2.2%
Ser (S)  16	  7.1%
Thr (T)  10	  4.4%
Trp (W)   0	  0.0%
Tyr (Y)   9	  4.0%
Val (V)  12	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1118
Hydrogen    H	      1788
Nitrogen    N	       284
Oxygen      O	       331
Sulfur      S	         5

Formula: C1118H1788N284O331S5
Total number of atoms: 3526

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13535
Abs 0.1% (=1 g/l)   0.549, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.544, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.56
This classifies the protein as stable.



Aliphatic index: 107.96

Grand average of hydropathicity (GRAVY): 0.142