ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPINIRRATI NDIICMQNAN LHNLPENYMM KYYMYHILSW PEASFVATTT TLDCEDSDEQ 

        70         80         90        100        110        120 
DENDKLELTL DGTNDGRTIK LDPTYLAPGE KLVGYVLVKM NDDPDQQNEP PNGHITSLSV 

       130        140        150        160        170        180 
MRTYRRMGIA ENLMRQALFA LREVHQAEYV SLHVRQSNRA ALHLYRDTLA FEVLSIEKSY 

       190        200        210        220        230 
YQDGEDAYAM KKVLKLEELQ ISNFTHRRLK ENEEKLEDDL ESDLLEDIIK QGVNDIIV 

References and documentation are available.

Number of amino acids: 238

Molecular weight: 27603.17

Theoretical pI: 4.77

Amino acid composition: 
Ala (A)  14	  5.9%
Arg (R)  13	  5.5%
Asn (N)  16	  6.7%
Asp (D)  20	  8.4%
Cys (C)   2	  0.8%
Gln (Q)  10	  4.2%
Glu (E)  22	  9.2%
Gly (G)   8	  3.4%
His (H)   7	  2.9%
Ile (I)  15	  6.3%
Leu (L)  28	 11.8%
Lys (K)  12	  5.0%
Met (M)  10	  4.2%
Phe (F)   4	  1.7%
Pro (P)   8	  3.4%
Ser (S)  11	  4.6%
Thr (T)  13	  5.5%
Trp (W)   1	  0.4%
Tyr (Y)  12	  5.0%
Val (V)  12	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1208
Hydrogen    H	      1912
Nitrogen    N	       330
Oxygen      O	       385
Sulfur      S	        12

Formula: C1208H1912N330O385S12
Total number of atoms: 3847

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23505
Abs 0.1% (=1 g/l)   0.852, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.847, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.56
This classifies the protein as unstable.



Aliphatic index: 90.97

Grand average of hydropathicity (GRAVY): -0.552