ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPISEPTWAE FPFFFKSAVV ELLPYKNRCC LRKCSKPNQF LVDSTPNHLH DVAIHTCENY 

        70         80         90        100        110        120 
TVFTYSDSPD TVFTLKYLKN IGENDLKIEY SESSVLFVPG KNQKFIFKTS IHVENVAADL 

       130        140        150        160        170        180 
RLIVVLMLEP LENLWRIADL GGFWRSMRRT YPSRVFVGDT FCYWSGMTLA VVSILGHFSK 

       190        200        210        220        230        240 
ILMLFFIPQI INFLYSIPQL FHLVPCPRHR LPKYDPKTDT VSMSVAEFKK TDLKRVGALF 

       250        260        270        280        290        300 
ITICKSIGML HVKEVEKDGE IYLQINNLTI INLVLKFAGP LHEKTLNDVL MSIQILCSLL 

       310 
AFFIRFYLAS LFYDVVE 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 36591.05

Theoretical pI: 8.33

Amino acid composition: 
Ala (A)  12	  3.8%
Arg (R)  12	  3.8%
Asn (N)  14	  4.4%
Asp (D)  14	  4.4%
Cys (C)   8	  2.5%
Gln (Q)   6	  1.9%
Glu (E)  16	  5.0%
Gly (G)  11	  3.5%
His (H)   9	  2.8%
Ile (I)  24	  7.6%
Leu (L)  39	 12.3%
Lys (K)  21	  6.6%
Met (M)   8	  2.5%
Phe (F)  25	  7.9%
Pro (P)  17	  5.4%
Ser (S)  22	  6.9%
Thr (T)  17	  5.4%
Trp (W)   4	  1.3%
Tyr (Y)  12	  3.8%
Val (V)  26	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1700
Hydrogen    H	      2628
Nitrogen    N	       416
Oxygen      O	       449
Sulfur      S	        16

Formula: C1700H2628N416O449S16
Total number of atoms: 5209

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40380
Abs 0.1% (=1 g/l)   1.104, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   1.090, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.79
This classifies the protein as stable.



Aliphatic index: 105.08

Grand average of hydropathicity (GRAVY): 0.228