ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPISSHSHSG QFCLHAQGKL EDVIQEAIQQ GFQSFSFTEH TPRDRVEDLY PEELHLQPED 

        70         80         90        100        110        120 
LFKTFDEYVN EARRLKQNYG DQINILIGAE TEYIRPESVE LLKSLNTKYN LDYFVGSVHH 

       130        140        150        160        170        180 
VNSIPIDFSP ELWQKALQHV GNNPEQLFID YFEHQYDLMQ RLHPLVIGHF DLICLFAPED 

       190        200        210        220        230        240 
AKEVFKNSKS VWELIQRNIK YAVSYGGIFE INTSAFRKGW KTAYPQQRLL ELMVEQGAQL 

       250        260        270        280        290        300 
TLSDDSHGPH QVGLNYHLAK SYLDKCGITS LCMIEKGPDG NGTVVKNVTV DNVWSKFVGT 


NGITNT 

References and documentation are available.

Number of amino acids: 306

Molecular weight: 35050.47

Theoretical pI: 5.41

Amino acid composition: 
Ala (A)  12	  3.9%
Arg (R)   9	  2.9%
Asn (N)  17	  5.6%
Asp (D)  17	  5.6%
Cys (C)   4	  1.3%
Gln (Q)  20	  6.5%
Glu (E)  23	  7.5%
Gly (G)  20	  6.5%
His (H)  14	  4.6%
Ile (I)  19	  6.2%
Leu (L)  30	  9.8%
Lys (K)  17	  5.6%
Met (M)   4	  1.3%
Phe (F)  16	  5.2%
Pro (P)  13	  4.2%
Ser (S)  20	  6.5%
Thr (T)  14	  4.6%
Trp (W)   4	  1.3%
Tyr (Y)  13	  4.2%
Val (V)  20	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1579
Hydrogen    H	      2405
Nitrogen    N	       419
Oxygen      O	       471
Sulfur      S	         8

Formula: C1579H2405N419O471S8
Total number of atoms: 4882

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41620
Abs 0.1% (=1 g/l)   1.187, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   1.180, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.00
This classifies the protein as stable.



Aliphatic index: 85.33

Grand average of hydropathicity (GRAVY): -0.421