ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPISVDKAVI ARLKVHGETF EILVDPYLAR DFKEGKEVPI EEILATPYVF KDAHKGDKAS 

        70         80         90        100        110        120 
EKEMEKIFGT SDPYEVAKII LRKGEVQLTA QQRREMLEEK KRQIATIIHR HAVDPRTGYP 

       130        140        150        160        170        180 
HPVDRILRAM EEVGVRVDIF KDAEAQVQDV IKAIRRILPL RIEMKVIAVK IPSEYVGRAY 

       190        200        210        220        230 
GEVRKFGRIK KEEWASDGSW LFLIEIPGGV EEEFYEKLNA LTKGNAQTKL IERKGL 

References and documentation are available.

Number of amino acids: 236

Molecular weight: 27062.47

Theoretical pI: 8.54

Amino acid composition: 
Ala (A)  19	  8.1%
Arg (R)  18	  7.6%
Asn (N)   2	  0.8%
Asp (D)  12	  5.1%
Cys (C)   0	  0.0%
Gln (Q)   7	  3.0%
Glu (E)  28	 11.9%
Gly (G)  15	  6.4%
His (H)   5	  2.1%
Ile (I)  23	  9.7%
Leu (L)  16	  6.8%
Lys (K)  24	 10.2%
Met (M)   5	  2.1%
Phe (F)   8	  3.4%
Pro (P)  11	  4.7%
Ser (S)   6	  2.5%
Thr (T)   8	  3.4%
Trp (W)   2	  0.8%
Tyr (Y)   7	  3.0%
Val (V)  20	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1221
Hydrogen    H	      1977
Nitrogen    N	       335
Oxygen      O	       347
Sulfur      S	         5

Formula: C1221H1977N335O347S5
Total number of atoms: 3885

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.792

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.76
This classifies the protein as stable.



Aliphatic index: 97.08

Grand average of hydropathicity (GRAVY): -0.392