ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPIWKTQTFF TSISVIQIVN KETKVSTKKE KDSMLNQLNT ILRFLFLFLQ LIKSSAAVEP 

        70         80         90        100        110        120 
NGGPNILDHN IMLVNTNATI PKKEQTDFEV ISPTKQTQVD EDCKKGLYHI ENAGNLIELQ 

       130        140        150        160        170        180 
AKCWKVVGNI EISSNYSGSL IDLGLIREIE GDLIIKNNKH IFRIQGYNLE SLGKLELDSL 

       190        200        210        220        230        240 
TSFVSLDFPA LKEVETVDWR VLPILSSVVI NGNIKKIKNI IISDTALTSI DYFNNVKKVD 

       250        260        270        280        290        300 
IFNINNNRFL ENLFASLESV TKQLTVHSNA KELELDLSNL HTVENMTIKD VSEIKLAKLS 

       310        320        330        340        350        360 
SVNSSLEFIE NQFSSLELPL LAKVQGTLGL IDNKNLKKLN FSNATDIQGG LMIANNTELA 

       370        380        390        400        410        420 
KIDFFPKLRQ IGGAIYFEGS FDKIDLPELK LVKGSAYIKS SSEELNCEEF TSPKAGRSII 

       430        440        450        460        470        480 
RGGKIECTSG MKSKMLNVDE EGNVLGKQET DNDNGKKEKG KNGAKSQGSS KKMENSAPKN 

       490        500 
IFIDAFKMSV YAVFTVLFSI IF 

References and documentation are available.

Number of amino acids: 502

Molecular weight: 55939.40

Theoretical pI: 6.59

Amino acid composition: 
Ala (A)  21	  4.2%
Arg (R)   8	  1.6%
Asn (N)  44	  8.8%
Asp (D)  24	  4.8%
Cys (C)   4	  0.8%
Gln (Q)  16	  3.2%
Glu (E)  37	  7.4%
Gly (G)  29	  5.8%
His (H)   5	  1.0%
Ile (I)  49	  9.8%
Leu (L)  55	 11.0%
Lys (K)  52	 10.4%
Met (M)   9	  1.8%
Phe (F)  26	  5.2%
Pro (P)  12	  2.4%
Ser (S)  46	  9.2%
Thr (T)  26	  5.2%
Trp (W)   3	  0.6%
Tyr (Y)   7	  1.4%
Val (V)  29	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2506
Hydrogen    H	      4049
Nitrogen    N	       651
Oxygen      O	       764
Sulfur      S	        13

Formula: C2506H4049N651O764S13
Total number of atoms: 7983

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27180
Abs 0.1% (=1 g/l)   0.486, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.481, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.06
This classifies the protein as unstable.



Aliphatic index: 101.73

Grand average of hydropathicity (GRAVY): -0.173