ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKFPQFRLI LVLFYLISMI QWSVITFSLG FFLNVCIFAY FVFFKSLPDL PKPQPRFVDI 

        70         80         90        100        110        120 
VPESSNTVDV DKELKSVEGL IQDGNAQIGK ELESIVNLII KDFVQPWFTK IDKNSDAEFL 

       130        140        150        160        170        180 
KVIKWRLLQT LLVVKDKLMK NDSASLIVLK LLPIFNKHFS TFCDAREAVL SDLTLERHKS 

       190        200        210        220        230        240 
ANIDLQIAVE FNKNYKIHKS LSLKPNALQK EIEKSIRKTV IGLLPHLFDN DELDSLLVFT 

       250        260        270        280        290        300 
LMTEVLTTCI ISPLIFKFTD PDSWNLRIVS LSQNYFEEKH KVHKIRRMLS KELQDHRKVM 

       310        320        330        340        350        360 
NDVANKDVGE PSSEKLELNA EYTGKQFEHY LNQLDSLLDL SDIKYVAYSL ALKIYQLKEN 

       370        380        390        400        410        420 
EHLTKENLKY KKRLLLSLNL IESKLSFPGS EIDTASKKLA REANYPDLNM DNGIVLKEMA 

       430        440        450        460        470        480 
SFLTSITLKD IVDDSEFLPF FESFLGSVPE TQGSTFLEYS QTIESFKNPL EDATSEDIIS 

       490        500        510        520        530        540 
GYSGISTMQL QEISSKFFHN NNLQNMKLLD EGLVKNIILF RNSFQINNDE DTFILARKSV 

       550        560        570        580        590        600 
LLLQTEAIKY LDDRFLPLFK KTPSFLKMLS TSHIISTDIY AHFLSRIGGV NNPEQNKIIK 

       610        620        630        640        650        660 
DNVKTDFMNP VRIFANPGIT DALDNIVNGS GSKPHKSRIS SNPRYSQLFG SENDNIFKDK 

       670        680        690        700        710        720 
LFDDENDNTS EISVVEDQLD HPRNMEKVSV SSGNSGLNPS QFYGSNNFRD NIASLTISID 

       730        740        750        760        770        780 
QIEKELELLR HLILKADLTN NQMQLKILKK SQRTLLKELE MKELLKQQYM VQENGNSLFR 

       790        800        810        820        830        840 
KTKIYIRSYF SENSSNGLKE ITYYIINIHH FNNGQVSSWD MARRYNEFFE LNTYLKKNFR 

       850        860        870        880        890        900 
DLMRQLQDLF PSKVKMSLKY HVTKTLLYEE RKQKLEKYLR ELLSISEICE DNIFRRFLTD 

       910        920        930        940        950        960 
PTPFKLNKEY MHDDILEEPL HEPIGSSNST SNSSSVVDLQ SSEDGGELNF YEDERHFFTD 

       970        980        990       1000       1010       1020 
SGYPFYSQNK SFVKQICDLF ISLFALNKAN AGWLRGRAII TVLQQLLGST IEKYIKVSIQ 

      1030       1040       1050       1060       1070       1080 
KLRSEDQVFE AIVTFKNMLW GDNGLFERKR NETAEATRSE GERLRTEQLA LTSLQRLFAD 

      1090       1100       1110       1120 
TCGRVVGLRD SHEAAGRVHA MLQNPYLNAS LLLEALDAIL LDIICND 

References and documentation are available.

Number of amino acids: 1127

Molecular weight: 129980.85

Theoretical pI: 6.01

Amino acid composition: 
Ala (A)  41	  3.6%
Arg (R)  46	  4.1%
Asn (N)  78	  6.9%
Asp (D)  71	  6.3%
Cys (C)   7	  0.6%
Gln (Q)  46	  4.1%
Glu (E)  81	  7.2%
Gly (G)  39	  3.5%
His (H)  23	  2.0%
Ile (I)  83	  7.4%
Leu (L) 148	 13.1%
Lys (K)  92	  8.2%
Met (M)  22	  2.0%
Phe (F)  69	  6.1%
Pro (P)  35	  3.1%
Ser (S) 103	  9.1%
Thr (T)  50	  4.4%
Trp (W)   7	  0.6%
Tyr (Y)  33	  2.9%
Val (V)  53	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 152
Total number of positively charged residues (Arg + Lys): 138

Atomic composition:

Carbon      C	      5859
Hydrogen    H	      9248
Nitrogen    N	      1534
Oxygen      O	      1742
Sulfur      S	        29

Formula: C5859H9248N1534O1742S29
Total number of atoms: 18412

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    88045
Abs 0.1% (=1 g/l)   0.677, assuming all pairs of Cys residues form cystines


Ext. coefficient    87670
Abs 0.1% (=1 g/l)   0.674, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.46
This classifies the protein as unstable.



Aliphatic index: 97.21

Grand average of hydropathicity (GRAVY): -0.318