ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKIKKPKKN GPPPEGYSKI EPTLTKYRNK LKSAQANPDP TKSKQSSLWI IYQLNYKITR 

        70         80         90        100        110        120 
YVYDTYVAKR ISKELYDWLL LQNDINKDLI AKWKKPGYEK LCCINCISTN TNGGGTCVCR 

       130        140        150 
VPKAKLLEKD PEKVNIECIT CGCRGCASSD 

References and documentation are available.

Number of amino acids: 150

Molecular weight: 17052.92

Theoretical pI: 9.41

Amino acid composition: 
Ala (A)   6	  4.0%
Arg (R)   5	  3.3%
Asn (N)  10	  6.7%
Asp (D)   7	  4.7%
Cys (C)   9	  6.0%
Gln (Q)   4	  2.7%
Glu (E)   7	  4.7%
Gly (G)   8	  5.3%
His (H)   0	  0.0%
Ile (I)  12	  8.0%
Leu (L)  12	  8.0%
Lys (K)  23	 15.3%
Met (M)   1	  0.7%
Phe (F)   0	  0.0%
Pro (P)  11	  7.3%
Ser (S)   9	  6.0%
Thr (T)   9	  6.0%
Trp (W)   3	  2.0%
Tyr (Y)   9	  6.0%
Val (V)   5	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	       758
Hydrogen    H	      1227
Nitrogen    N	       205
Oxygen      O	       220
Sulfur      S	        10

Formula: C758H1227N205O220S10
Total number of atoms: 2420

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30410
Abs 0.1% (=1 g/l)   1.783, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.754, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.68
This classifies the protein as stable.



Aliphatic index: 76.07

Grand average of hydropathicity (GRAVY): -0.687