ProtParam
User-provided sequence:
10 20 30 40 50 60
MPKKLAKLIE NSQLYRKQTE KCKKSRRTDV LQTDASNCIF IDRSPKHFDL ILNFLRDGDV
70 80 90 100 110 120
DLPEQGKELK EPRLLIEYGK ILDLVEYQRL GFNVSDIMER YGNQLHIAFQ KNSDPEECKS
130 140 150 160 170
TLQFKTNIRA ISQRYDSWQY SNANKQFTKF VDDSLAFIAD YADNEDDSEN DTQNADHN
References and
documentation are available.
Number of amino acids: 178
Molecular weight: 20851.28
Theoretical pI: 5.30
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 915
Hydrogen H 1432
Nitrogen N 256
Oxygen O 292
Sulfur S 5
Formula: C
915H
1432N
256O
292S
5
Total number of atoms: 2900
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16055
Abs 0.1% (=1 g/l) 0.770, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.764, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.97
This classifies the protein as unstable.
Aliphatic index: 74.55
Grand average of hydropathicity (GRAVY): -0.897