ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKKVWKSST PSTYEHISSL RPKFVSRVDN VLHQRKSLTF SNVVVPDKKN NTLTSSVIYS 

        70         80         90        100        110        120 
QGSDIYEIDF AVPLQEAASE PVKDYGDAFE GIENTSLSPK FVYQGETVSK MAYLDKTGET 

       130        140        150        160        170        180 
TLLSMSKNGS LAWFKEGIKV PIHIVQELMG PATSYASIHS LTRPGDLPEK DFSLAISDFG 

       190        200        210        220        230        240 
ISNDTETIVK SQSNGDEEDS ILKIIDNAGK PGEILRTVHV PGTTVTHTVR FFDNHIFASC 

       250        260        270        280        290        300 
SDDNILRFWD TRTSDKPIWV LGEPKNGKLT SFDCSQVSNN LFVTGFSTGI IKLWDARAAE 

       310        320        330        340        350        360 
AATTDLTYRQ NGEDPIQNEI ANFYHAGGDS VVDVQFSATS SSEFFTVGGT GNIYHWNTDY 

       370        380        390        400        410        420 
SLSKYNPDDT IAPPQDATEE SQTKSLRFLH KGGSRRSPKQ IGRRNTAAWH PVIENLVGTV 

       430 
DDDSLVSIYK PYTEESE 

References and documentation are available.

Number of amino acids: 437

Molecular weight: 48382.74

Theoretical pI: 5.17

Amino acid composition: 
Ala (A)  23	  5.3%
Arg (R)  15	  3.4%
Asn (N)  22	  5.0%
Asp (D)  32	  7.3%
Cys (C)   2	  0.5%
Gln (Q)  13	  3.0%
Glu (E)  26	  5.9%
Gly (G)  28	  6.4%
His (H)  11	  2.5%
Ile (I)  28	  6.4%
Leu (L)  27	  6.2%
Lys (K)  27	  6.2%
Met (M)   4	  0.9%
Phe (F)  20	  4.6%
Pro (P)  22	  5.0%
Ser (S)  49	 11.2%
Thr (T)  37	  8.5%
Trp (W)   7	  1.6%
Tyr (Y)  14	  3.2%
Val (V)  30	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      2148
Hydrogen    H	      3315
Nitrogen    N	       573
Oxygen      O	       689
Sulfur      S	         6

Formula: C2148H3315N573O689S6
Total number of atoms: 6731

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59485
Abs 0.1% (=1 g/l)   1.229, assuming all pairs of Cys residues form cystines


Ext. coefficient    59360
Abs 0.1% (=1 g/l)   1.227, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.60
This classifies the protein as unstable.



Aliphatic index: 74.26

Grand average of hydropathicity (GRAVY): -0.469